Molecular and General Genetics MGG

, Volume 185, Issue 1, pp 132–135 | Cite as

Partial characterization of 5-fluoropyrimidine-resistant mutants of Neurospora crassa

  • Frank P. Buxton
  • Alan Radford
Article

Summary

The primary lesion in a number of 5-fluoropyrimidine resistant mutants of Neurospora crassa has been identified. ud-1 mutants, previously designated fdu-2, are deficient in nucleoside uptake and show extensive intragenic complementation. uc-4 mutants lack uracil phosphoribosyl transferase with no complementation between 23 alleles. udk mutants lack uridine kinase activity. fdu-2 mutants affect the repression of the first two de novo pyrimidine biosynthetic enzymes, have no detectable uridine kinase activity and show decreased uridine uptake. Accordingly, fdu-2 may be involved in the regulation of pyrimidine uptake, salvage and de novo synthesis.

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References

  1. Beckwith JR, Pardee AB, Austrian R, Jacob F (1962) Co-ordination of the synthesis of the enzymes in the pyrimidine pathway of Escherichia coli. J Mol Biol 5: 618–634Google Scholar
  2. Buxton, FB, Radford A (1982) Nitrogen metabolite repression of fluoropyrimidine resistance and pyrimidine uptake in Neurospora crassa. Mol Gen Genet, in pressGoogle Scholar
  3. Buxton FB, Radford A (1982) Isolation and mapping of fluoropyrimidine-resistant mutants of Neurospora crassa. Mol Gen Genet 185: 129–131Google Scholar
  4. Dalke P, Magill JM (1979) Specificity of uracil uptake in Neurospora crassa. J Bacteriol 139: 212–219Google Scholar
  5. De Serres FJ (1966) The utilisation of leaky ad-3 mutants of Neurospora crassa in heterokaryon tests for allelic complementation. Mutant Res 3: 3–12Google Scholar
  6. Dunaway-Mariano D, Magill JM (1978) Specificity of nucleoside transport in Neurospora crassa. J Bacteriol 136: 924–928Google Scholar
  7. Griswold WR, Madrid VO, Shaffer PM, Tappen DC, Pugh CSG, Abbott MT (1976) Regulation of thymidine metabolism in Neurospora crassa. J Bacteriol 125: 1040–1047Google Scholar
  8. Hoffmann GR, Malling HV, Mitchell TJ (1973) Genetics of 5-fluoropyrimidine-resistant mutants in Neurospora crassa. Can J Genet Cytol 15: 831–844Google Scholar
  9. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1952) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  10. Magill JM, Edwards ES, Sabina RL, Magill CW (1976) Depression of uracil uptake by ammonium in Neurospora crassa. J Bacteriol 127: 1265–1269Google Scholar
  11. O'Donovan GA, Neuhard J (1970) Pyrimidine metabolism in micro-organisms. Bacteriol Rev 34: 278–343Google Scholar
  12. Prescott LM, Jones ME (1969) Modified methods for the determination of carbamoyl aspartate. Anal Biochem 32: 408–419Google Scholar
  13. Pynadath TI, Fink RM (1967) Studies of orotidine 5-monophosphate decarboxylase in Neurospora crassa. Arch Biochem Biophys 118: 185–189Google Scholar
  14. Shaffer PM, Hsu CA, Abbott MT (1975) Metabolism of deoxyribonucleosides in Neurospora crassa. J Bacteriol 121: 648–655Google Scholar
  15. Williams LG, Davis RH (1970) Pyrimidine specific carbamyl phosphate synthetase in Neurospora crassa. J Bacteriol 103: 335–341Google Scholar
  16. Williams LG, Mitchell HK (1969) Mutants affecting thymidine metabolism in Neurospora crassa. J Bacteriol 100: 383–389Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Frank P. Buxton
    • 1
  • Alan Radford
    • 1
  1. 1.Department of GeneticsLeeds UniversityLeedsUK

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