Molecular and General Genetics MGG

, Volume 185, Issue 1, pp 129–131 | Cite as

Isolation and mapping of fluoropyrimidine-resistant mutants of Neurospora crassa

  • Frank P. Buxton
  • Alan Radford
Article

Summary

Growth characteristics of wild type Neurospora crassa on 5-fluorouracil, 5-fluorouridine and 5-fluorodeoxyuridine and methods for selecting mutants resistant to these pyrimidine analogues are described. The mutants are mapped and characterized for resistance to the analogues. Some mutants are found to be allelic with the previously described ud-1 (fdu-1) ad uc-4 genes.

References

  1. Dunaway-Mariano D, Magill JM (1978) Specificity of nucleoside transport in Neurospora crassa. J Bacteriol 136: 924–928Google Scholar
  2. Griswold WR, Madrid VO, Shaffer PM, Tappen DC, Pugh CSG, Abbott MT (1976) Regulation of thymidine metabolism in Neurospora crassa. J Bacteriol 125: 1040–1047Google Scholar
  3. Hoffmann GR, Malling HV, Mitchell TJ (1973) Genetics of 5-fluoropyrimidine-resistant mutants of Neurospora crassa. Can J Genet Cytol 15: 831–844Google Scholar
  4. Lacroute F (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95: 824–832Google Scholar
  5. Magill JM, Edwards ES, Sabina RL, Magill CW (1976) Depression of uracil uptake by ammonium in Neurospora crassa. J Bacteriol 127: 1265–1269Google Scholar
  6. Makoff AJ, Radford A (1976) The location of the feedback-specific region within the pyrimidine-3-locus of Neurospora crassa. Mol Gen Genet 146: 247–251Google Scholar
  7. Palmer LM, Scazzocchio C, Cove DJ (1975) Pyrimidine biosynthesis in Aspergillus nidulans: Isolation and characterisation of mutants resistant to fluoropyrimidines. Mol Gen Genet 140: 165–173Google Scholar
  8. Radford A (1969) Polarised complementation at the pyrimidine-3-locus of Neurospora. Mol Gen Genet 104: 288–294Google Scholar
  9. Shaffer PM, Hsu CA, Abbott MT (1975) Metabolism of deoxyribonucleosides in Neurospora crassa. J Bacteriol 121: 648–655Google Scholar
  10. Williams LG, Mitchell HK (1969) Mutants affecting thymidine metabolism in Neurospora crassa. J Bacteriol 100: 383–389Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Frank P. Buxton
    • 1
  • Alan Radford
    • 1
  1. 1.Department of GeneticsLeeds UniversityLeedsUK

Personalised recommendations