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Molecular and General Genetics MGG

, Volume 185, Issue 1, pp 105–110 | Cite as

Cloning of ompF, the structural gene for an outer membrane pore protein of E. coli K12: Physical localization and homology with the phoE gene

  • Jan Tommassen
  • Peter van der Ley
  • Arie van der Ende
  • Hans Bergmans
  • Ben Lugtenberg
Article

Summary

The ompF gene of Escherichia coli K12, which is the structural gene for an outer membrane pore protein has been cloned into the multicopy plasmid vector pACYC184. Expression of the cloned ompF gene results in overproduction of the OmpF protein and in strongly decreased production of the other two pore proteins, OmpC protein and PhoE protein, whereas production of OmpA protein is much less affected. Expression of the cloned ompF gene is still dependent on the ompB+ gene product, but is hardly influenced by the osmolarity of the growth medium. The gene was localized on the hybrid plasmids by the analysis of in vitro constructed deletion plasmids and mutant plasmids generated by Tn5 insertions. Heteroduplex analysis revealed an extensive DNA homology of the ompF gene with the phoE gene. Since the direction of transcription of the phoE gene is known, the direction of transcription of the ompF gene could be deduced.

Keywords

Structural Gene Plasmid Vector Mutant Plasmid Multicopy Plasmid Hybrid Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Jan Tommassen
    • 1
  • Peter van der Ley
    • 1
  • Arie van der Ende
    • 1
  • Hans Bergmans
    • 1
  • Ben Lugtenberg
    • 1
  1. 1.Department of Molecular Cell Biology, Section Microbiology and Institute for Molecular BiologyState UniversityUtrechtThe Netherlands

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