Molecular and General Genetics MGG

, Volume 185, Issue 1, pp 43–50 | Cite as

Constitutive expression of SOS functions and modulation of mutagenesis resulting from resolution of genetic instability at or near the recA locus of Escherichia coli

  • Evelyn M. Witkin
  • J. Owen McCall
  • Michael R. Volkert
  • Ingbritt E. Wermundsen
Article

Summary

Cellular activities normally inducible by DNA damage (SOS functions) are expressed, without DNA damage, in recA441 (formerly tif-1) mutants of Escherichia coli at 42° C but not at 30° C. We describe a strain (SC30) that expresses SOS functions (including mutator activity, prophage induction and copious synthesis of recA protein) constitutively at both temperatures. SC30 is one of four stable subclones (SC strains) derived from an unstable recombinant obtained in a conjugation between a recA441 K12 donor and a recA+ B/r-derived recipient. SC30 does not owe its SOS-constitutive phenotype to a mutation in the lexA gene (which codes the repressor of recA and other DNA damage-inducible genes), since it is lexA+. Each of the SC strains expresses SOS functions in a distinctively anomalous way. We show that the genetic basis for the differences in SOS expression among the SC strains is located at or very near the recA locus. We propose that resolution of genetic instability in this region, in the original recombinant, has altered the pattern of expression of SOS functions in the SC strains.

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References

  1. Anderson RP, Roth JR (1977) Tandem genetic duplications in phage and bacteria. Annu Rev Microbiol 31:473–505Google Scholar
  2. Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44, 1–56Google Scholar
  3. Bagg A, Kenyon CJ, Walker GC (1981) Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci USA 78:5749–5753Google Scholar
  4. Brent R, Ptashne M (1980) The lexA gene product represses its own promoter. Proc Natl Acad Sci USA 77:1932–1936Google Scholar
  5. Brent R, Ptashne M (1981) Mechanism of action of the lexA gene product. Proc Natl Acad Sci USA 78:4204–4208Google Scholar
  6. Castellazzi M, George J, Buttin G (1972a) Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Mol Gen Genet 119:139–152Google Scholar
  7. Castellazzi M, George J, Buttin G (1972b) Prophage induction and cell division in E. coli. II. Linked (recA, zab) and unlinked (lex) suppressors of tif-1-mediated induction and filamentation. Mol Gen Genet 119:153–174Google Scholar
  8. Clark AJ, Margulies AD (1965) Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc Natl Acad Sci USA 53:451–459Google Scholar
  9. Craig NL, Roberts JW (1980) E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotide. Nature 283:26–30Google Scholar
  10. Craig NL, Roberts JW (1981) Function of nucleoside triphosphate and polynucleotide in Escherichia coli recA protein-directed cleavage of phage λ repressor. J Biol Chem 256:8039–8044Google Scholar
  11. Emmerson PT, West SC (1977) Identification of protein X of Escherichia coli as the recA +/tif + gene product. Mol Genet 155:77–85Google Scholar
  12. Gudas LJ, Mount DW (1977) Identification of the recA(tif) gene product of Escherichia coli. Proc Natl Acad Sci USA 74:5280–5284Google Scholar
  13. Gudas LJ, Pardee AB (1975) Model for the regulation of Escherichia coli DNA repair functions. Proc Natl Acad Sci USA 72:2330–2334Google Scholar
  14. Hill RF (1965) Ultraviolet-induced lethality and reversion to protorophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem Photobiol 4:563–568Google Scholar
  15. Huisman O, D'Ari R (1981) An inducible DNA replication-cell division coupling mechanism in E. coli. Nature 290:797–799Google Scholar
  16. Irbe RM, Morin LME, Oishi M (1981) Prophage (ϕ80) induction in Escherichia coli K-12 by specific deoxyoligonucleotides. Proc Natl Acad Sci USA 78:138–142Google Scholar
  17. Kenyon CJ, Walker GC (1980) DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci USA 77:2819–2823Google Scholar
  18. Kenyon CJ, Walker GC (1981) Expression of the E. coli uvrA gene is inducible. Nature 289:808–810Google Scholar
  19. Kirby EP, Ruff WL, Goldthwait DA (1972) Cell division and prophage induction in Escherichia coli: effects of pantoyl lactone and various furan derivatives. J Bacteriol 111:447–453Google Scholar
  20. Kogoma T, Torrey TA, Connaughton MJ (1979) Induction of UV-resistant DNA replication in Escherichia coli: induced stable DNA replication as an SOS function. Mol Gen Genet 176:1–9Google Scholar
  21. Laemmli UK, Favre M (1973) Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol 80:575–599Google Scholar
  22. Little JW, Edmiston SH, Pacelli LZ, Mount DW (1980) Cleavage of the Escherichia coli lexA protein by the recA protease. Proc Natl Acad Sci USA 77:3225–3229Google Scholar
  23. Little JW, Harper JE (1979) Identification of the lexA gene product of Escherichia coli K-12. Proc Natl Acad Sci USA 76:6147–6151Google Scholar
  24. Little JW, Mount DW, Yanisch-Perron CR (1981) Purified lexA protein is a repressor of the recA and lexA genes. Proc Natl Acad Sci USA 78:4199–4203Google Scholar
  25. Lloyd RG (1978) Hyper-recombination in Escherichia coli K-12 mutants constitutive for protein X synthesis. J Bacteriol 134:929–935Google Scholar
  26. McEntee K (1977) Protein X is the product of the recA gene of Escherichia coli. Proc Natl Acad Sci USA 74:5275–5279Google Scholar
  27. McEntee K, Weinstock GM, Lehman IR (1980) recA protein-catalyzed strand assimilation: stimulation by Escherichia coli single-strand DNA-binding protein. Proc Natl Acad Sci USA 77:857–861Google Scholar
  28. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  29. Morand P, Blanco M, Devoret R (1977) Characterization of lexB mutations in Escherichia coli K-12. Bacteriol 131:512–582Google Scholar
  30. Mount DW (1977) A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc Natl Acad Sci USA 74:300–304Google Scholar
  31. Pacelli LZ, Edmiston SH, Mount DW (1979) Isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. J Bacteriol 137:568–573Google Scholar
  32. Roberts JW, Roberts CW, Craig NL (1978) Escherichia coli recA gene product inactivates phage λ repressor. Proc Natl Acad Sci USA 75:4714–4718Google Scholar
  33. Sancar A, Stachelek C, Konigsberg W, Rupp WD (1980) Sequences of the recA gene and protein. Proc Natl Acad Sci USA 77:2611–2615Google Scholar
  34. Shibata T, DasGupta C, Cunningham RP, Radding CM (1980) Homologous pairing in genetic recombination: formation of D loops by combined action of recA protein and a helix-destabilizing protein. Proc Natl Acad Sci USA 77:2606–2610Google Scholar
  35. Sussman R, Resnick J, Calame K, Baluch J (1978) Interaction of bacteriophage λ repressor with non operator DNA containing single-strand gaps. Proc Natl Acad Sci USA 75:5817–5821Google Scholar
  36. Volkert MR, Margossian LJ, Clark AJ (1981) Evidence that rnmB is the operator of the Escherichia coli recA gene. Proc Natl Acad Sci USA 78:1786–1790Google Scholar
  37. Witkin EM (1974) Thermal enhancement of ultraviolet mutability in a tif-1 uvr A derivative of Escherichia coli B/r: evidence that ultraviolet mutagenesis depends upon an inducible function. Proc Natl Acad Sci USA 71:1930–1934Google Scholar
  38. Witkin EM (1975) Persistence and decay of thermoinducible errorprone repair activity in nonfilamentous derivatives of tif-1 Escherichia coli B/r: the timing of some critical events in ultraviolet mutagenesis. Mol Gen Genet 142:87–103Google Scholar
  39. Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907Google Scholar
  40. Witkin EM, Kirschmeier P (1978) Complexity in the regulation of SOS functions in bacteria. In: DNA repair mechanisms Hanawalt PC, Friedberg EC, Fox CF (eds) Academic Press, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Evelyn M. Witkin
    • 1
  • J. Owen McCall
    • 1
  • Michael R. Volkert
    • 2
  • Ingbritt E. Wermundsen
    • 1
  1. 1.Department of Biological Sciences, Douglass CampusRutgers UniversityNew BrunswickUSA
  2. 2.Department of Molecular BiologyUniversity of CaliforniaBerkeleyUSA

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