Molecular and General Genetics MGG

, Volume 206, Issue 2, pp 200–206 | Cite as

Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology

  • Mark A. Estelle
  • Chris Somerville


Mutant lines of Arabidopsis thaliana resistant to the artificial auxin 2,4-dichloro phenoxyacetic acid (2,4-D) were isolated by screening for growth of seedlings in the presence of toxic levels of 2,4-D. Genetic analysis of these resistant lines indicated that 2,4-D resistance is due to a recessive mutation at a locus we have designated Axr-1. Mutant seedlings were resistant to approximately 50-fold higher concentrations of 2,4-D than wild-type and were also resistant to 8-fold higher concentrations of indole-3-acetic acid (IAA) than wild-type. Labelling studies with (14C)2,4-D suggest that resistance was not due to changes in uptake or metabolism of 2,4-D. In addition to auxin resistance the mutants have a distinct morphological phenotype including alterations of the roots, leaves, and flowers. Genetic evidence indicates that both auxin resistance and the morphological changes are due to the same mutation. Because of the pleiotropic morphological effects of these mutations the Axr-1 gene may code for a function involved in auxin action in all tissues of the plant.

Key words

Auxin resistance 2,4-Dichloro phenoxyacetic acid Phytohormone Plant development Herbicide-resistance 


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  1. Bearder JR (1980) Plant hormones and other growth substances — their background, structures and occurrence. Encyclopedia of Plant Physiol 9:9–80Google Scholar
  2. Blakely LM, Radaway SJ, Hollen LB, Croker SG (1972) Control and kinetics of branch root formation in cultured root segments of Haplopoppus ravenii. Plant Physiol 50:35–42Google Scholar
  3. Browse J, McCourt P, Somerville CR (1985) A mutant of Arabidopsis lacking a chloroplast-specific lipid. Science 227:763–765Google Scholar
  4. Caspar T, Huber SC, Somerville C (1985) Alterations in growth, photosynthesis and respiration in a starchless mutant of Arabidopsis thaliana (L.) deficient in chloroplast phosphoglucomutase activity. Plant Physiol 79:11–17Google Scholar
  5. Chaleff RS, Parsons MF (1978) Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum. Proc Natl Acad Sci USA 75:5104–5107Google Scholar
  6. Davidonis GH, Hamilton RH, Mumma RO (1982) Metabolism of 2,4-dichlorophenoxyacetic acid in 2,4-dichlorophenoxyacetic acid-resistant soybean callus tissue. Plant Physiol 70:104–107Google Scholar
  7. Estelle MA, Somerville CR (1986) The mutants of Arabidopsis Trends in Genetics 2:89–93Google Scholar
  8. Feung C, Hamilton RH, Mumma RO (1975) Metabolism of 2,4-dichlorophenoxyacetic acid. VII. Comparison of metabolites from five species of plant cellus tissue cultures. J Agric Food Chem 23:373–376Google Scholar
  9. Jacobs M, Ray P (1976) Rapid auxin-induced decrease in free space pH and its relationship to auxin-induced growth in maize and pea. Plant Physiol 58:203–209Google Scholar
  10. Koning RE (1983) The roles of auxin, ethylene, and acid growth in filament elongation in Gaillardia grandiflora. Am J Bot 70:602–610Google Scholar
  11. Koorneef M, Reuling G, Karssen CM (1984) The isolation and characterization of abscissic acid-insensitive mutants of Arabidopsis thaliana. Plant Physiol 61:377–383Google Scholar
  12. Koorneef M, Elgersma A, Hanhart CJ, Van Loenen-Martinet EP, van Rign L, Zeevaart JAD (1985) A gibberellin insensitive mutant of Arabidopsis thaliana. Plant Physiol 64:35–39Google Scholar
  13. Maher EP, Martindale SJB (1980) Mutants of Arabidopsis thaliana with altered responses to auxins and gravity. Biochem Genet 18:1041–1053Google Scholar
  14. Meyerowitz EM, Pruitt RE (1985) Arabidopsis thaliana and plant molecular genetics. Science 229:1214–1218Google Scholar
  15. Mirza JI, Maher EP (1980) More 2,4-D resistant mutants. Arabidopsis Inf Serv 17:103–107Google Scholar
  16. Mirza JI, Olsen GM, Iversen TH, Maher EP (1984) The growth and gravitropic responses of wild-type and auxin-resistant mutants of Arabidopsis thaliana. Plant Physiol 60:516–522Google Scholar
  17. Moore R, Smith JD (1985) Graviresponsiveness and abscissic-acid content of roots of carotenoid-deficient mutants of Zea mays L. Planta 164:126–128Google Scholar
  18. Muller JF, Goujaud J, Caboche M (1985) Isolation in vitro of napthaleneacetic acid-tolerant mutants of Nicotiana tabacum, which are impaired in root morphogenesis. Mol Gen Genet 199:194–200Google Scholar
  19. Phillips ID (1975) Apical dominance. Annu Rev Plant Physiol 26:341–367Google Scholar
  20. Phinney BO, Gibberellin A (1984) Dwarfisn and the control of shoot elongation in higher plants In: Crozier A, Hillman JB (eds) The biosynthesis and metabolism of plant hormones. Soc Exp Biol Semin Series 23. Cambridge University Press, Cambridge, pp 17–42Google Scholar
  21. Shininger TL (1979) The control of vascular development. Annu Rev Plant Physiol 30:313–337Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Mark A. Estelle
    • 1
  • Chris Somerville
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA

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