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Molecular and General Genetics MGG

, Volume 126, Issue 1, pp 53–69 | Cite as

Isolation and characterization of a ribonuclease III deficient mutant of Escherichia coli

  • Peter Kindler
  • Till Uwe Keil
  • Peter Hans Hofschneider
Article

Summary

A mutant of E. coli K 12 AB301 RNAase 19 - , selected for its inability to degrade double-stranded RNA, has been isolated and shown to have less than 1% of RNAase III-activity related to the parental strain.

Keywords

Parental Strain Deficient Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

TCA

trichloracetic acid

RF

replicative form of phage-RNA

Enzymes

Lysozyme

(E.C. 3.2.1.17)

RNAase

(E.C. 2.7.7.16)

DNAase

(E.C. 3.1.4.5)

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References

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Peter Kindler
    • 1
  • Till Uwe Keil
    • 1
  • Peter Hans Hofschneider
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried bei MünchenGermany

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