Molecular and General Genetics MGG

, Volume 202, Issue 3, pp 493–499 | Cite as

Organization of transcription units around the Drosophila melanogaster rudimentary locus and temporal pattern of expression

  • J. M. Rawls
  • J. -N. Freund
  • B. P. Jarry
  • C. Louis
  • W. A. Segraves
  • P. Schedl
Article
Received:

Summary

The molecular organization of 90 kb of DNA derived from a region of the X chromosome that encompasses the rudimentary locus of D. melanogaster is presented. This segment spans the cytogenetic region 14F2-3 to 15A1-2, and there are, in addition to the rudimentary gene several transcription units present, whose functions are still unknown. We have determined the pattern of expression of all these genes at several stages of development, and found that they all show a different temporal modulation of their activity. The accumulation of the r product correlated well with the enzymatic activity determined for the protein product of the gene, being highest in very early embryos and adult females.

Key words

Northern blots Polytene chromosomes Gene expression 
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References

  1. Ambrosio L, Schedl P (1984) Gene expression during Drosophila melanogaster oogenesis: Analysis by in situ hybridization to tissue sections. Dev Biol 105:80–92Google Scholar
  2. Bridges CB (1935) Salivary chromosome maps. J Hered 26:60–64Google Scholar
  3. Brothers V, Tsubota S, Germeraad S, Fristrom J (1978) The rudimentary locus of Drosophila melanogaster: partial purification of a carbamyl phosphate synthetase—aspartate transcarbamylase —dihydroorotase complex. Biochem Genet 16:321–332Google Scholar
  4. Carlson PS (1971) A genetic analysis of the rudimentary locus of Drosophila melanogaster. Genet Res 17:53–81Google Scholar
  5. Falk D, Boselli L, Curtiss S, Halladay D, Klufas C (1984) The characterization of chromosome breaks in Drosophila melanogaster. I. Mass isolation of deficiencies which have an endpoint in the 14A–15A region. Mutat Res 126:25–34Google Scholar
  6. Hall LMC, Mason PJ, Spierer P (1983) Transcripts, genes and bands in 315000 base-pairs of Drosophila DNA. J Mol Biol 169:83–96Google Scholar
  7. Jarry BP (1978) Purification of aspartate transcarbamylase from Drosophila melanogaster. Eur J Biochem 87:533–540Google Scholar
  8. Farry BP (1979) Genetical and cytological location of the structural parts coding for the first three steps of pyrimidine biosynthesis in Drosophila melanogaster. Mol Gen Genet 172:199–202Google Scholar
  9. Jarry BP, Falk DR (1974) Functional diversity within the rudimentary locus of Drosophila melanogaster. Mol Gen Genet 135:113–120Google Scholar
  10. McGinnis W, Levine MS, Hafen E, Kuroiwa A, Gehring WJ (1984) A conserved DNA sequence in homeiotic genes of the Drosophila antennapedia and bithorax complexes. Nature (London) 308:428–433Google Scholar
  11. McNight SL, Miller OL (1976) Ultrastructural patterns of RNA synthesis during embryogenesis of Drosophila melanogaster. Cell 8:305–319Google Scholar
  12. Morgan TH (1910) The method of inheritance of two sex-limited characters in the same animal. Proc Soc Exp Biol Med 8:17–19Google Scholar
  13. Norby S (1973) The biochemical genetics of rudimentary mutants of Drosophila melanogaster. Hereditas 73:11–16Google Scholar
  14. Rawls JM (1979) The enzymes of de novo pyrimidine biosynthesis in Drosophila melanogaster: their isolation, properties and expression druing development. Comp Biochem Physiol 62B:207–216Google Scholar
  15. Rawls JM, Fristrom JW (1975) A complex genetic locus that controls the first three steps of pyrimidine biosynthesis in Drosophila. Nature (London) 255:738–740Google Scholar
  16. Rawls JM, Porter LA (1979) Organization of the rudimentary wing locus in Drosophila melanogaster: the isolation and partial characterization of mutants induced with ethyl methane sulfonate, ICR-170 and X-rays. Genetics 93:143–161Google Scholar
  17. Rigby JW, Dieckman M, Rhodes C, Berg P (1977) Labelling deoxynucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Mol Biol 113:237–252Google Scholar
  18. Segraves WA, Louis C, Schedl P, Jarry BP (1983) Isolation of the rudimentary locus of Drosophila melanogaster. Mol Gen Genet 189:34–40Google Scholar
  19. Segraves WA, Louis C, Tsubota S, Schedl P, Rawls JM, Jarry BP (1984) The rudimentary locus of Drosophila melanogaster. J Mol Biol 175:1–17Google Scholar
  20. Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  21. Spierer P, Spierer A, Bender W, Hogness DS (1983) Molecular mapping of genetic and chromomeric units in Drosophila melanogaster. J Mol Biol 168:35–50Google Scholar
  22. Steward R, McNally FJ, Scheld P (1984) Isolation of the dorsal locus of Drosophila. Nature (London) 311:262–265Google Scholar
  23. Tsubota S (1980) A genetic and biochemical analysis of the rudimentary locus in Drosophila melanogaster. Ph.D. Thesis, University of California, BerkeleyGoogle Scholar
  24. Wharton KA, Yedvobnick B, Finnerty VG, Artavanis-Tsakonas S (1985) opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in Drosophila melanogaster. Cell 40:55–62Google Scholar
  25. Zalokar M (1976) Autoradiographic study of protein and RNA formation during early development of Drosophila eggs. Dev Biol 49:425–437Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. M. Rawls
    • 1
  • J. -N. Freund
    • 2
  • B. P. Jarry
    • 2
  • C. Louis
    • 3
  • W. A. Segraves
    • 4
  • P. Schedl
    • 5
  1. 1.T.H. Morgan School of Biological SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Laboratoire de Génetique Moléculaire des Eucarvotes du CNRSStrasbourg, CedexFrance
  3. 3.Institute of Molecular Biology and Biotechnology, Research Center of Crete and Department of BiologyUniversity of CreteCreteGreece
  4. 4.Department of Biochemistry, School of MedicineStanford UniversityStanfordUSA
  5. 5.Department of BiologyPrinceton UniversityPrincetonUSA

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