Molecular and General Genetics MGG

, Volume 176, Issue 3, pp 411–415 | Cite as

Sporulation of mitochondrial respiratory deficient mit- mutants of Saccharomyces cerevisiae

  • Elke Pratje
  • Reinhard Schulz
  • Susanne Schnierer
  • Georg Michaelis
Article

Summary

The role of mitochondrial protein synthesis, electron transport, and four specific mitochondrial gene products on sporulation were studied in respiratory deficient mit- mutants. These mutants were isolated in an op1 strain and localized on the mitochondrial genome by petite deletion mapping. All 153 mutations studied could be assigned to the four mitochondrial regions OXI1, OXI2, OXI3 and COB, known to affect cytochrome c oxidase and cytochrome b. The specific loss of one mitochondrially translated polypeptide was found in some mutants of each locus: OXI1—cytochrome c oxidase subunit 2, OXI2 — subunit 3, OXI3 — subunit 1, and COB — cytochrome b.

The ability of diploid mit- mutants to sporulate was systematically investigated. About one third of the mutants, representing three loci, were incapable of forming spores. All other cultures produced either respiratory competent mit+ tetrads, both mit+ and mit- tetrads, or only mit- tetrads. Mutants forming mit- tetrads mapped in all four loci. These results demonstrate that in contrast to petite mutants some mit- mutants have retained the ability to perform meiosis and sporulation.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexander, N.J., Vincent, R.D., Perlman, P.S., Miller, D.H., Hanson, D.K., Mahler, H.R.: Regulatory interactions between mitochondrial genes. I. Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase. J. Biol. Chem. 254, 2471–2479 (1979)Google Scholar
  2. Bonner, W.M., Laskey, R.A.: A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46, 83–88 (1974)Google Scholar
  3. Cabral, F., Solioz, M., Rudin, Y., Schatz, G., Clavilier, L., Slonimski, P.P.: Identification of the structural gene for yeast cytochrome c oxidase subunit II on mitochondrial DNA. J. Biol. Chem. 253, 297–304 (1978)Google Scholar
  4. Claisse, M.L., Spyridakis, A., Slonimski, P.P.: Mutations at any one of three unlinked mitochondrial genetic loci BOX1, BOX4, and BOX6, modify the structure of cytochrome b polypeptide(s). In: Mitochondria 1977, Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F. (eds.), pp. 337–344. Berlin: de Gruyter 1977Google Scholar
  5. Diala, E.S., Wilkie, D.: Mitochondria — cell relationships in Saccharomyces cerevisiae: aspects of sporulation. In: Mitochondria 1977, Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F. (eds.), pp. 563–570. Berlin: de Gruyter 1977Google Scholar
  6. Douglas, M.G., Butow, R.A.: Variant forms of mitochondrial translation products in yeast: evidence for location of determinants on mitochondrial DNA. Proc. Natl. Acad. Sci. U.S.A. 73, 1083–1086 (1976)Google Scholar
  7. Esposito, M.S., Esposito, R.E.: Mutants of meiosis and ascospore formation. In: Methods in cell biology, Prescott, D.M. (ed.), Vol. XI, pp. 303–326. New York: Academic Press 1975Google Scholar
  8. Fowell, R.R.: Sporulation and hybridization of yeasts. In: The Yeasts, Rose, A.H., Harrison, J.S. (eds.), Vol. I, pp. 303–383. London and New York: Academic Press 1969Google Scholar
  9. Haber, J.E., Halvorson, H.O.: Methods in sporulation and germination of yeasts. In: Methods in cell biology, Prescott, D.M. (ed.), Vol. XI, pp. 45–69. New York: Academic Press 1975Google Scholar
  10. Haid, A., Schweyen, R.J., Bechmann, H., Kaudewitz, F., Solioz, M., Schatz, G.: The mitochondrial COB region in yeast codes for apocytochrome b and is mosaic. Eur. J. Biochem. 94, 451–464 (1979)Google Scholar
  11. Kotylak, Z., Slonimski, P.P.: Mitochondrial mutants isolated by a new screening method based upon the use of the nuclear mutation opl. In: Mitochondria 1977, Bandlow, W., Schweyen, R.J., Wolf, K., Kaudewitz, F. (eds.), pp. 83–89. Berlin: de Gruyter 1977Google Scholar
  12. Kovacova, V., Irmlerova, J., Kovac, L.: Oxidative phosphorylation in yeast. IV. Combination of a nuclear mutation affecting oxidative phosphorylation with cytoplasmic mutation to respiratory deficiency. Biochim. Biophys. Acta 162, 157–163 (1968)Google Scholar
  13. Küenzi, M.T., Tingle, M.A., Halvorson, H.O.: Sporulation of Saccharomyces cerevisiae in the absence of a functional mitochondrial genome. J. Bacteriol. 117, 80–88 (1974)Google Scholar
  14. Newlon, M.C., Hall, B.D.: Inhibition of yeast sporulation by ethidium bromide. Mol. Gen. Genet. 165, 113–114 (1978)Google Scholar
  15. Petersen, J.G., Olson, L.W., Zickler, D.: Synchronous sporulation of Saccharomyces cerevisiae at high cell concentrations. Carlsberg Res. Commun. 43, 241–253 (1978)Google Scholar
  16. Pratje, E., Michaelis, G.: Allelism studies of mitochondrial mutants resistant to antimycin A or funiculosin in Saccharomyces cerevisiae. Mol. Gen. Genet. 152, 167–174 (1977)Google Scholar
  17. Putrament, A., Baranowska, H., Prazmo, W.: Induction by manganese of mitochondrial antibiotic resistant mutations in yeast. Mol. Gen. Genet. 126, 357–366 (1973)Google Scholar
  18. Slonimski, P.P., Tzagoloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Eur. J. Biochem. 61, 27–41 (1976)Google Scholar
  19. Tzagoloff, A., Akai, A., Needleman, R.B.: Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase. Proc. Natl. Acad. Sci. U.S.A. 72, 2054–2057 (1975)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Elke Pratje
    • 1
  • Reinhard Schulz
    • 1
  • Susanne Schnierer
    • 1
  • Georg Michaelis
    • 1
  1. 1.Fakultät für BiologieUniversität BielefeldBielefeld 1Federal Republic of Germany

Personalised recommendations