Molecular and General Genetics MGG

, Volume 198, Issue 3, pp 432–436 | Cite as

Generation of deletions through a cis-acting mutation in plasmid pC194

  • Juan Carlos Alonso
  • Thomas A. Trautner


When plasmid pC194-1 is ligated to pBR322 to generate plasmid pHV15-1, deletions occur with high frequency within the joined pBR322 DNA. Generation of deletions is recE4 independent, and occurs in B. subtilis with a 1,000-fold higher frequency than in Escherichia coli. In the hybrid plasmid pVH15-1, deletion end-points are not at random, but at defined locations within pBR322. We propose that the base alteration, characterizing pC194-1, has stabilized within the plasmid a stem/loop structure, which acts as a deletion generator.


Escherichia Coli Deletion Generator Hybrid Plasmid Base Alteration Plasmid pC194 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Juan Carlos Alonso
    • 1
  • Thomas A. Trautner
    • 1
  1. 1.Max-Planck-Institut für molekulare GenetikBerlin 33

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