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Molecular and General Genetics MGG

, Volume 198, Issue 3, pp 432–436 | Cite as

Generation of deletions through a cis-acting mutation in plasmid pC194

  • Juan Carlos Alonso
  • Thomas A. Trautner
Article

Summary

When plasmid pC194-1 is ligated to pBR322 to generate plasmid pHV15-1, deletions occur with high frequency within the joined pBR322 DNA. Generation of deletions is recE4 independent, and occurs in B. subtilis with a 1,000-fold higher frequency than in Escherichia coli. In the hybrid plasmid pVH15-1, deletion end-points are not at random, but at defined locations within pBR322. We propose that the base alteration, characterizing pC194-1, has stabilized within the plasmid a stem/loop structure, which acts as a deletion generator.

Keywords

Escherichia Coli Deletion Generator Hybrid Plasmid Base Alteration Plasmid pC194 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Juan Carlos Alonso
    • 1
  • Thomas A. Trautner
    • 1
  1. 1.Max-Planck-Institut für molekulare GenetikBerlin 33

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