Molecular and General Genetics MGG

, Volume 195, Issue 1–2, pp 170–174 | Cite as

Genetic inactivation of topoisomerase I suppresses a defect in initiation of chromosome replication in Escherichia coli

  • Jacqueline Louarn
  • Jean-Pierre Bouché
  • Josette Patte
  • Jean-Michel Louarn
Article
Received:

Summary

A strain of Escherichia coli K12 harboring simultaneously the temperature-sensitive dnaA46 mutation and a deletion of the trp-topA-cysB region plates with the same full efficiency at 30° C and 42° C. We have analyzed the possible involvement of the gene coding for topoisomerase I, topA, in this suppression phenomenon. The Ts phenotype was retrieved upon introduction of a plasmid-borne DNA fragment including an active topA gene into this strain, but not upon introduction of the same fragment harboring a topA::Tn1000 insertion. Replication seems to remain DnaA-dependent in the Δ(topA) strain, however, since we have been unable to introduce a dnaA::Tn10 allele. We propose either that the dnaA46 gene product is overproduced and compensates for its thermal inactivation, or that initiation at oriC demands less DnaA protein in the absence of topoisomerase I.

Keywords

Escherichia Coli Gene Product dnaA46 Mutation Thermal Inactivation dnaA46 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Apr

ampicillin resistance

Cmr

chloramphenicol resistance

Kmr

Kanamycin resistance

Str

streptomycin resistance

Tcr

tetracycline resistance

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jacqueline Louarn
    • 1
  • Jean-Pierre Bouché
    • 1
  • Josette Patte
    • 1
  • Jean-Michel Louarn
    • 1
  1. 1.Centre de Recherche de Biochimie et de Génétique cellulaires du C.N.R.S.Toulouse CedexFrance

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