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Molecular and General Genetics MGG

, Volume 195, Issue 1–2, pp 101–107 | Cite as

Behaviour of the transposons Tn5 and Tn7 in Xanthomonas campestris pv. campestris

  • Peter Turner
  • Christine Barber
  • Michael Daniels
Article

Summary

Xanthomonas campestris pv. campestris was tested for its ability to maintain various plasmids after they had been transferred by conjugation from Escherichia coli donors. Broad host-range plasmids belonging to incompatibility groups P and Q could be maintained but X. campestris was unable to support replication of narrow host-range ColE1, pACYC184 and pBR325 replicons. Delivery systems based on E. coli donors of suicide plasmids and on X. campestris Hfrs were used to introduce Tn7 and Tn5 into X. campestris. Tn7 insertions were recovered at high frequency while Tn5 transposed at low frequency. Three auxotrophic Tn5 insertions were isolated but transposition of Tn7 into the X. campestris genome did not generate any auxotrophs. DNA hybridization analysis showed that Tn7 had inserted into the same “hot spot(s)” in all cases tested.

Keywords

Delivery System Hybridization Analysis Xanthomonas Campestris Incompatibility Group Suicide Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Peter Turner
    • 1
  • Christine Barber
    • 1
  • Michael Daniels
    • 1
  1. 1.John Innes InstituteColney LaneEngland

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