Molecular and General Genetics MGG

, Volume 188, Issue 2, pp 169–172 | Cite as

An estimate of the global error frequency in translation

  • Nathan Ellis
  • Jonathan Gallant


Electrophoretic heterogeneity in a set of selected proteins is used to estimate the average error frequency during translation. Estimates based upon streptomycin-induced heterogeneity as well as mistranslation of an ochre codon yield an average error frequency of 4x10-4 for normally growing cells.


Codon Average Error Global Error Error Frequency Electrophoretic Heterogeneity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bloch PL, Phillips TS, Neidhardt FC (1980) Protein identification on O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol 141:1409–1420Google Scholar
  2. Copeland BR, Todd SA, Furlong CE (1982) High resolution two-dimensional electrophoresis of human erythrocyte membrane proteins. Am J Human Genet 34:15–31Google Scholar
  3. Davies J, Gilert W, Gorini L (1964) Streptomycin, suppression, and the code. Proc Natl Acad Sci USA 51:883–890Google Scholar
  4. Davies J, Gorini L, Davis B (1965) Misreading of RNA code words induced by amino glycoside antibiotics. Mol Pharmacol 1:93–106Google Scholar
  5. Davies J, Jones DS, Khorana HG (1966) A further study of mis-reading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. J Mol Biol 18:48–57Google Scholar
  6. Edelmann P, Gallant J (1977) Mistranslation in E. coli. Cell 10:131–137Google Scholar
  7. Gallant J, Palmer L (1979) Error propagation in viable cells. Mech Ageing Dev 10:27–28Google Scholar
  8. Gorini L (1974) Streptomycin and misreading of the genetic code. In: Nomura M, Tissieres A, Lengyel P (eds) Ribosomes. Cold Spring Harbor Laboratory, New York, p 791Google Scholar
  9. Kurland CG, Gallant J (1982) The secret life of the ribosome. In: Galas D (ed) Accuracy in biology. Marcel Dekker, New York, (in press)Google Scholar
  10. Loftfield R, Vanderjagt D (1972) The frequency of errors in protein synthesis. Biochem J 128:1353–1356Google Scholar
  11. O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins J Biol Chem 250:4007–4021Google Scholar
  12. O'Farrell PH (1978) The suppression of defective translation by ppGpp and its role in the stringent response. Cell 15:545–557Google Scholar
  13. Parker J, Ollard JW, Friesen JD, Stanner CP (1978) Stuttering: high level mistranslation in animal and bacterial cells. Proc Natl Acad Sci USA 75:1091–1095Google Scholar
  14. Parker J, Flanagan J, Murphy J, Gallant J (1981) On the accuracy of protein synthesis in Drosophila melanogaster. Mech Ageing Dev 16:127–139Google Scholar
  15. Parker J, Friesen JD (1980) “Two out of three” codon reading leading to mistranslation in vivo. Mol Gen Genet 177:439–445Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Nathan Ellis
    • 1
  • Jonathan Gallant
    • 1
  1. 1.Department of GeneticsUniversity of WashingtonSeattleUSA

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