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Molecular and General Genetics MGG

, Volume 165, Issue 3, pp 239–245 | Cite as

Somatic hybrids of plants by fusion of protoplasts

Observations on the model system “Nicotiana glauca-Nicotiana langsdorffii
  • Yves Chupeau
  • Claudine Missonier
  • Marie-Christine Hommel
  • Jacques Goujaud
Article

Summary

Fusions of protoplasts from Nicotiana langsdorffii and Nicotiana glauca were induced using polyethylene glycol. Parasexual hybrid colonies were selected for their ability to grow without growth substances. Hybrid plants, regenerated after grafting, were all tumorous and exhibited morphological and chromosome number variations. Out of 48 colonies selected in vitro only 6 regenerated flowering plants. Two of these plants had 42 chromosomes and were morphologically identical to the sexual amphidiploid Nicotiana glaucaxlangsdorffii.

Keywords

Polyethylene Glycol Chromosome Number Number Variation Flowering Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Yves Chupeau
    • 1
  • Claudine Missonier
    • 1
  • Marie-Christine Hommel
    • 1
  • Jacques Goujaud
    • 1
  1. 1.Laboratoire de Biologie cellulaireInstitut National de la Recherche AgronomiqueVersaillesFrance

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