An electron microscopic study of the development of rat testis in the first 10 postnatal days

  • Anna M. Novi
  • Paolo Saba


The ultrastructure of testis of immature rats from birth to ten postnatal days was studied in order to describe the development of germ cells into definitive spermatogonia. The primordial germ cells showed no structural changes from birth to 4 days after birth. At the 5th postnatal day the gonocytes were transformed into darker cells and began to migrate towards the basement membrane. As they reached the periphery of the seminiferous cords they resumed mitoses and gave rise to smaller gonocytes, which were progressively transformed in definitive spermatogonia. A lighter kind of supporting cell was also detected.


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  1. Allen, E.: Studies on degenerating sex cells in immature mammals. I. An analysis of degeneration in primordial and large germ cells in male albino rats aged 1–9 days. J. Morph. 85, 405–421 (1949).Google Scholar
  2. Baillie, A. H.: The histochemistry and ultrastructure of the gonocyte. J. Anat. (Lond.) 98, 641–645 (1964).Google Scholar
  3. Beaumont, H. M., and A. M. Mandl: A quantitative study of primordial germ cells in the male rat. J. Embryol. exp. Morph. 11, 715–740 (1963).Google Scholar
  4. Clermont, Y., and B. Perey: Quantitative study of the cell population of the seminiferous tubules in immature rats. Amer. J. Anat. 100, 241–267 (1957).Google Scholar
  5. Flickinger, Ch. J.: The postnatal development of the Sertoli cells of the mouse. Z. Zellforsch. 78, 92–113 (1967).Google Scholar
  6. Franchi, L. L., and A. M. Mandl: The ultrastructure of germ cells in foetal and neonatal male rats. J. Embryol. exp. Morph. 12, 289–308 (1964).Google Scholar
  7. Hargitt, G. T.: The formation of the sex glands and germ cells of mammals. II. The history of the male germ cells in the albino rat. J. Morph. 42, 253–305 (1926).Google Scholar
  8. Hoven, H.: Histogénèse du testicule des mammifères. Anat. Anz. 47, 90–109 (1914).Google Scholar
  9. Huckins, C.: Changes in gonocytes at the time of initiation of spermatogenesis in the rat. Anat. Rec. 145, 243 (1963).Google Scholar
  10. Karnovsky, M. J.: Simple methods for “Staining with lead” at high pH in electron microscopy. J. biophys. biochem. Cytol. 11, 729–732 (1961).Google Scholar
  11. Kirkham, W. B.: The germ cell cycle in the mouse. Anat. Rec. 10, 217–219 (1915).Google Scholar
  12. Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).Google Scholar
  13. Mancini, R. E., R. Narbaitz, and J. C. Lavieri: Origin and development of the germinative epithelium and Sertoli cells in the human testis: cytological, cytochemical and quantitative study. Anat. Rec. 136, 477–490 (1960).Google Scholar
  14. Widmaier, R.: Über die postnatale Hodenentwicklung und Keimzellreifung bei der Maus. Z. mikr.anat. Forsch. 70, 215–241 (1963).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Anna M. Novi
    • 1
  • Paolo Saba
    • 1
  1. 1.Istituto di Patologia GeneralePisaItalia

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