Chromosoma

, Volume 46, Issue 1, pp 13–22 | Cite as

Cytogenetic studies of chromosome replication in Zea mays L.: Regulation of homologue synchrony

  • George R. Douglas
  • David B. Walden
Article
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Abstract

Maize stocks with or without additional heterochromatin (a B chromosome or abnormal chromosome 10), and a stock heteromorphic for a reciprocal translocation, were used in a study of homologue synchrony of replication. Batches of attached primary roots were pulse labelled with 3H-TdR and sampled at hourly intervals during the S period (at 18° C). Silver grain counts over each chromosome arm from 25 cells at each collection period were submitted to analyses. Qualitative synchrony (concordance) was tested between chromosome arms using a χ2 test. Quantitative synchrony was tested with an I-divergence test. Results from these tests in the control experiments were consistent with the hypothesis of meta-synchrony of homologue replication. The addition of heterochromatin in the form of a B chromosome or an abnormal 10 chromosome increased asynchrony two-fold. The insertion of a heteromorphic translocation into the genome produced a similar though less pronounced effect.

Keywords

Control Experiment Developmental Biology Abnormal Chromosome Pronounced Effect Primary Root 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • George R. Douglas
    • 1
    • 2
    • 3
  • David B. Walden
    • 1
    • 2
  1. 1.Department of GeneticsHealth Sciences CenterWinnipeg
  2. 2.Department of Plant SciencesUniversity of Western OntarioLondon
  3. 3.Human Development Division Department of Health and WelfareEnvironmental Health Center Tunney's PastureOttawaCanada

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