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Molecular and General Genetics MGG

, Volume 216, Issue 1, pp 75–80 | Cite as

Cloning and sequencing of the Pseudomonas aeruginosa 1244 pilin structural gene

  • Peter A. Castric
  • Hazel F. Sidberry
  • Jerald C. Sadoff
Article

Summary

The pilin structural gene of Pseudomonas aeruginosa 1244 was cloned in both cosmids and lambda. Expression of the cloned gene was detected in P. aeruginosa strains PAO2003, PA103, and 653A by an immunoblot reaction utilizing monoclonal antibodies. Western blot analysis showed that pilin expressed from the cloned gene was slightly larger than native 1244 pilin when produced in strains PAO2003 and 653A, but distinctly smaller in PA103. Bacteriophages specific for the 1244 pilus did not lyse strain PAO2003 containing the cloned 1244 pilin gene, indicating that functional 1244 pili were not assembled in this recombinant strain. Nucleotide sequencing revealed a coding region which when translated would produce a 15615 dalton peptide. The amino-terminal region of this peptide is identical with published pilin sequences. While the rest of the peptides are generally dissimilar, common residues are seen within potentially antigenic regions.

Key words

Pseudomonas aeruginosa Pili Pilin Virulence factor Adherence factor 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Peter A. Castric
    • 1
  • Hazel F. Sidberry
    • 2
  • Jerald C. Sadoff
    • 2
  1. 1.Department of Biological SciencesDuquesne UniversityPittsburghUSA
  2. 2.Department of Bacterial DiseasesWalter Reed Army Institute of ResearchWashington, DCUSA

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