Fresenius' Journal of Analytical Chemistry

, Volume 336, Issue 7, pp 589–592 | Cite as

Application of differential pulse polarography for trace determination of polycytidylic acid in absence and presence of some metal ions

  • Y. M. Temerk
  • M. M. Kamal
  • Z. A. Ahmed
  • M. S. Ibrahim
Original Papers Organic Analysis

Summary

A differential pulse polarographic method has been applied to the determination of trace concentrations of polycytidylic acid (Poly-C) in absence and presence of metal ions. The applicability of differential pulse polarography for the trace determination of the investigated biological compound was examined with regard to the dependence of differential pulse current on various parameters such as pH, pulse amplitude, scan rate and drop time. The selectivity of this technique for the determination of binary and a ternary mixtures of poly-C and some metal ions has been also reported. Limits of detection and quantitation have been calculated for the differential pulse polarographic determination of the poly-C and various metal ions. The validity of this method is supported by the constancy of the ip/C values and a statistical analysis is included on calibration curve parameters and observed concentration.

Keywords

Inorganic Chemistry Calibration Curve Pulse Current Pulse Amplitude Differential Pulse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Smith DL, Elving PJ (1962) J Am Chem Soc 84:1412Google Scholar
  2. 2.
    Smith DL, Elving PJ (1962) J Am Chem Soc 84:2741Google Scholar
  3. 3.
    Smith DL, Elving PJ (1962) Anal Chem 34:930Google Scholar
  4. 4.
    Janik B, Elving PJ (1968) Chem Rev 68:295Google Scholar
  5. 5.
    Dryhurst G (1972) Talanta 19:769Google Scholar
  6. 6.
    Janik B, Elving PJ (1973) J Am Chem Soc 95:99Google Scholar
  7. 7.
    Yao T, Wasa T, Musha S (1977) Bull Chem Soc Jpn 50:2917Google Scholar
  8. 8.
    Palecek E, Osteryoung J, Osteryoung RA (1982) Anal Chem 54:1389Google Scholar
  9. 9.
    Palecek E (1980) Anal Lett 13:331Google Scholar
  10. 10.
    Palecek E, Jelen F (1980) Collect Czech Chem Commun 45:3472Google Scholar
  11. 11.
    Palecek E (1980) Anal Biochem 108:189Google Scholar
  12. 12.
    Palecek E, Jelen F, Hung M (1981) Bioelectrochem Bioenerg 8:621Google Scholar
  13. 13.
    Cummings TE, Fraser JR, Elving PJ (1980) Anal Chem 52:558Google Scholar
  14. 14.
    Temerk YM, Kamal MM (1981) Fresenius Z Anal Chem 305:200Google Scholar
  15. 15.
    Temerk YM, Kamal MM, Ahmed ME, Ibrahim M (1983) Bioelectrochem Bioenerg 11:449Google Scholar
  16. 16.
    Temerk YM, Ahmed ZA, Ahmed ME, Kamal MM (1987) Fresenius Z Anal Chem 326:146Google Scholar
  17. 17.
    Christie JH, Osteryoung J, Osteryoung RA (1973) Anal Chem 45:210Google Scholar
  18. 18.
    Janik B, Elving PJ (1970) J Am Chem Soc 92:235Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. M. Temerk
    • 1
  • M. M. Kamal
    • 1
  • Z. A. Ahmed
    • 1
  • M. S. Ibrahim
    • 1
  1. 1.Chemistry Department, Faculty of ScienceAssiut UniversityAssiutEgypt

Personalised recommendations