Molecular and General Genetics MGG

, Volume 201, Issue 3, pp 558–564 | Cite as

Arrest of the mitotic cell cycle and of meiosis in Saccharomyces cerevisiae by MMS

  • Martin Kupiec
  • Giora Simchen


Methyl methane sulphonate (MMS) was found to arrest mitotic cells at a specific stage in the cell cycle. Reciprocal double shift experiments involving MMS and temperature shifts in several temperature-sensitive cell-cycle (cdc) mutants have located the MMS-sensitive stage after the cdc7 and cdc8 temperature-sensitive stages and before the cdc13, cdc5 and cdc14 stages. An interdependent relationship was found between the arrests caused by MMS, cdc40 and hydroxyurea. Marked increases in mitotic recombination were induced by MMS, both in diploid and haploid strains. Meiosis is arrested by MMS at a very early stage, before DNA replication.


Cell Cycle Recombination Saccharomyces Cerevisiae Saccharomyces Hydroxyurea 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Martin Kupiec
    • 1
  • Giora Simchen
    • 1
  1. 1.Department of GeneticsThe Hebrew UniversityJerusalemIsrael

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