Advertisement

Molecular and General Genetics MGG

, Volume 215, Issue 1, pp 161–164 | Cite as

The isolation and preliminary characterisation of a novel Escherichia coli mutant rorB with enhanced sensitivity to ionising radiation

  • Paul G. Debenham
  • Michael B. T. Webb
Article

Summary

Escherichia coli K803 cells were mutagenized and screened for the presence of clones sensitive to ψ-rays but not to ultraviolet light. One new mutant of this type, named rorB, was isolated. This mutant is both cross-sensitive to mitomycin C and shows reduced conjugal recombination frequencies, but to a lesser extent than the phenotypically similar mutant recN. Unlike previously reported mutants of E. coli or yeast with an enhanced sensitivity to ionising radiations, rorB appears to be near wild type in ability to rejoin DNA double-strand breaks. The rorB gene maps close to ilvGEDAC at 84.5 min of the E. coli chromosome.

Key words

Escherichia coli Ionising radiation Mutation DNA repair 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blocher D, Pohlit W (1982) DNA double strand breaks in Ehrlich ascites tumour cells at low doses of X-rays. II. Can cell death be attributed to double strand breaks? Int J Radiat Biol 42:329–338Google Scholar
  2. Bradley MO, Kohn KW (1979) X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Res 7:793–804Google Scholar
  3. Breckenridge L, Gorini L (1970) Genetic analysis of streptomycin resistance in Escherichia coli. Genetics 65:9–25Google Scholar
  4. Bryant PE (1984) Enzymatic restriction of mammalian cell DNA using PvuII and BamHI: evidence for the double strand break origin of chromosomal aberrations. Int J Radiat Biol 46:57–65Google Scholar
  5. Budd M, Mortimer RK (1982) Repair of double strand breaks in a temperature conditional radiation-sensitive mutant of Saccharomyces cerevisiae. Mutat Res 103:19–24Google Scholar
  6. Curtiss III R (1981) Gene transfer. In: Gerhardt P (ed) Manual of methods for general bacteriology. Washington, pp 243–265Google Scholar
  7. Debenham PG, Webb MBT, Law J (1988) The cloning of the rorB gene of Escherichia coli. Mol Gen Genet 215:156–160Google Scholar
  8. Frankenberg D, Frankenberg-Schwager M, Blocher D, Harbich R (1981) Evidence for DNA double strand breaks as the critical lesions in yeast cells irradiated with sparsely or densely ionizing radiation under oxic or anoxic conditions. Radiat Res 88:524–532Google Scholar
  9. Frankenberg D, Goodhead DT, Frankenberg-Schwager M, Harbich R, Bance DA, Wilkinson RE (1986) Effectiveness of 1.5 keV aluminium K and 0.3 keV carbon K characteristic X-rays at inducing DNA double strand breaks in yeast cells. Int J Radiat Biol 50:727–741Google Scholar
  10. Friedberg EC (1985) DNA Repair. Freeman, New YorkGoogle Scholar
  11. Giaccia A, Weinstein R, Hu J, Stamato T (1985) Cell cycle-dependent repair of double strand DNA breaks in a γ-ray sensitive Chinese hamster cell. Somat Cell Mol Genet 11:485–491Google Scholar
  12. Glickman BW, Zwenk H, Van Sluis EA, Rorsch A (1971) The isolation and characterisation of an X-ray sensitive ultraviolet-resistant mutant of Escherichia coli. Biochim Biophys Acta 254:144–154Google Scholar
  13. Glover SW (1962) Valine-resistant mutants of Escherichia coli K12. Genet Res 3:448–450Google Scholar
  14. Haas FL, Doudney CO (1957) A relation of nucleic acid synthesis to radiation-induced mutation frequency in bacteria. Proc Natl Acad Sci USA 43:871–883Google Scholar
  15. Joshi GP, Nelson WJ, Revell SH, Shaw CA (1982) X-ray induced chromosome damage in live mammalian cells, and improved measurements of its effects on their colony-forming ability. Int J Radiat Biol 41:161–181Google Scholar
  16. Kemp LM, Sedgwick SG, Jeggo PA (1984) X-ray sensitive mutants of Chinese hamster ovary cells defective in double strand break rejoining. Mutat Res 132:189–196Google Scholar
  17. Krasin F, Hutchinson F (1981) Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light. Proc Natl Acad Sci USA 78:3450–3453Google Scholar
  18. Natarajan AT, Obe G, van Zeeland AA, Palitti F, Meijers M, Verdegaal-Immerzeel EAM (1980) Molecular mechanisms in the production of chromosomal aberrations. Mutat Res 69:293–305Google Scholar
  19. Park MH, Wong BB, Lusk JE (1976) Mutants in three genes affecting transport of magnesium in Escherichia coli: Genetics and physiology. J Bacteriol 126:1096–1103Google Scholar
  20. Picksley SM, Attfield PV, Lloyd RG (1984) Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product. Mol Gen Genet 195:167–174Google Scholar
  21. Resnick MA, Martin P (1976) Repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Mol Gen Genet 143:119–129Google Scholar
  22. Russell RRB (1972) Mapping of a D-cycloserine resistance locus in Escherichia coli K12. J Bacteriol 111:622–624Google Scholar
  23. Wood WB (1966) Host specificity of DNA produced by E. coli: Bacterial mutants affecting the restriction and modification of DNA. J Mol Biol 16:118–133Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Paul G. Debenham
    • 1
  • Michael B. T. Webb
    • 1
  1. 1.Division of Cell and Molecular BiologyMRC Radiobiology UnitDidcotUK

Personalised recommendations