A general method to identify plant structural genes among genomic DNA clones using transposable element induced mutations
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Several genomic clones from Petroselinum hortense, Zea mays and Antirrhinum majus all homologous to cloned Petroselinum chalcone synthase cDNA were isolated using the λgt WES cloning system.
Clones containing the chalcone synthase structural gene were identified by hybridization to cDNA from Petroselinum hortense, genomic wildtype, mutant and revertant DNA.
Among the 5 different clones from Petroselinum hortense, PH3 is the most likely candidate to contain at least a portion of the chalcone synthase gene.
None of the 4 Zea mays clones appeared to contain part of the chalcone synthase gene.
Among the 2 different clones from Antirrhinum majus, AM3 contains the portion of the chalcone synthase structural gene which is altered in the mutant nivea recurrens (nivrec). This mutant is considered to be due to the integration of a transposable element. In revertants of nivrec to niv+ the wildtype locus is restored molecularly.
KeywordsStructural Gene Transposable Element Chalcone Genomic Clone Induce Mutation
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