Molecular and General Genetics MGG

, Volume 190, Issue 3, pp 403–405 | Cite as

Cloning of the structural gene for orotidine 5′-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli

  • F. P. Buxton
  • A. Radford
Article

Summary

A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5′-monophosphate carboxylase in E. coli is within the Neurospora sequence.

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References

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Copyright information

© Springer-Verlag GmbH & Co. KG 1983

Authors and Affiliations

  • F. P. Buxton
    • 1
  • A. Radford
    • 1
  1. 1.Department of GeneticsThe UniversityLeedsEngland

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