Molecular and General Genetics MGG

, Volume 204, Issue 3, pp 367–373 | Cite as

Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli

  • Jun-ichi Yamagishi
  • Hiroaki Yoshida
  • Michiko Yamayoshi
  • Shinichi Nakamura


DNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the first base of the 426th codon of the gyrB gene, resulting in an amino acid change from aspartic acid to asparagine, and nal-31 was an A to G transition at the first base of the 447th codon, resulting in an amino acid change from lysine to glutamic acid. This indicates that mutations in the gyrB gene are responsible for nalidixic acid resistance.

Key words

Nalidixic acid resistance gyrB gene Nucleotide sequence Escherichia coli 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Jun-ichi Yamagishi
    • 1
  • Hiroaki Yoshida
    • 1
  • Michiko Yamayoshi
    • 1
  • Shinichi Nakamura
    • 1
  1. 1.Research LaboratoriesDainippon Pharmaceutical Co. Ltd.Suita, OsakaJapan

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