Molecular and General Genetics MGG

, Volume 197, Issue 2, pp 345–346 | Cite as

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

  • Jef D. Boeke
  • Francois La Croute
  • Gerald R. Fink
Short Communication

Summary

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jef D. Boeke
    • 1
  • Francois La Croute
    • 2
  • Gerald R. Fink
    • 1
  1. 1.Massachusetts Institute of Technology and Whitehead Institute for Biomedical ResearchCambridgeUSA
  2. 2.Laboratoire de Genetique PhysiologiqueStrasbourgFrance

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