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Molecular and General Genetics MGG

, Volume 197, Issue 2, pp 345–346 | Cite as

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

  • Jef D. Boeke
  • Francois La Croute
  • Gerald R. Fink
Short Communication

Summary

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3 mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Keywords

Phosphate Pyrimidine Saccharomyces Cerevisiae Saccharomyces Mutant Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jef D. Boeke
    • 1
  • Francois La Croute
    • 2
  • Gerald R. Fink
    • 1
  1. 1.Massachusetts Institute of Technology and Whitehead Institute for Biomedical ResearchCambridgeUSA
  2. 2.Laboratoire de Genetique PhysiologiqueStrasbourgFrance

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