Molecular and General Genetics MGG

, Volume 199, Issue 3, pp 518–523 | Cite as

Rec-dependent and Rec-independent recombination of plasmid-borne duplication in Escherichia coli K12

  • Mark Matfield
  • Randa Badawi
  • William J. Brammar
Article

Summary

Plasmidic recombination in E. coli K12 has been previously demonstrated to be dependent on the host rec genotype. The construction of plasmids that carry a duplication within an antibiotic-resistance gene is described. Recombination between the direct repeats recreates an active antibiotic-resistance gene, allowing quantitative analysis of recombination frequencies in a closely related set of E. coli K12 strains carrying various rec mutations. Using this system, intraplasmidic recombination of a duplication within the pBR322 tetracycline-resistance gene is shown to be rec-dependent while recombination of a similar duplication within the kanamycin-resistance gene of Tn903 is shown to be independent of recA, recB, recC, recE, recF and sbcB.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Mark Matfield
    • 1
  • Randa Badawi
    • 2
  • William J. Brammar
    • 2
  1. 1.Dept. of MicrobiologyUniversity of LeicesterLeicesterGreat Britain
  2. 2.Dept. of BiochemistryUniversity of LeicesterLeicesterGreat Britain

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