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Molecular and General Genetics MGG

, Volume 211, Issue 2, pp 266–271 | Cite as

Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides

  • George W. Haughn
  • Julie Smith
  • Barbara Mazur
  • Chris Somerville
Article

Summary

A gene encoding acetolactate synthase was cloned from a chlorsulfuron-resistant mutant of Arabidopsis. The DNA sequence of the mutant gene differed from that of the wild type by a single base pair substitution. When introduced into tobacco by Ti plasmid-mediated transformation the gene conferred a high level of herbicide resistance. These results suggest that the cloned gene may confer agronomically useful levels of herbicide resistnace in other crop species, and that it may be useful as a selectable marker for plant transformation experiments.

Key words

Ti plasmid Chlorsulfuron Transformation Acetohydroxy acid synthase 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • George W. Haughn
    • 1
  • Julie Smith
    • 2
  • Barbara Mazur
    • 2
  • Chris Somerville
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  2. 2.Central Research and Development DepartmentWilmingtonUSA

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