The fine structure of the granulosa cells in the domestic fowl and the rat

  • Erik Dahl
Article
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Summary

The granulosa cells of the ovarian follicle of the rat and the domestic fowl have been studied with the light and electron microscope. The nuclei of the granulosa cells were irregular with indentations and large in proportion to the cytoplasm of the cell. The mitochondria had a dense, dark matrix with only few cristac. The Golgi apparatus was moderately developed, located towards the oocyte in a juxtanuclear position. The endoplasmic reticulum was rather sparse. Lipid droplets were only occasionally encountered. Microtubules were regularly observed. The functions of the granulosa cells are discussed. Compared with the steroid-producing cells of the theca interna of the same follicles, the granulosa cells primarily are the nursing cells for the growing oocyte and mainly have the characteristics of protein forming cells.

Key-Words

Ovary Rat Fowl Granulosa cells Ultrastructure 
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References

  1. Appelgren, L. E.: Sites of steroid hormone formation. Autoradiographic studies using labelled precursors. Acta physiol. scand., Suppl. 301, 1–108 (1967).Google Scholar
  2. Bjersing, L.: On the morphology and endocrine function of granulosa cells in ovarian follices and corpora lutea. Acta endocr. (Kbh.), Suppl. 125, 1–23 (1967).Google Scholar
  3. Björckman, N.: A study of the ultrastructure of the granulosa cells of the rat ovar. Acta anat. (Basel) 51, 125–147 (1962).Google Scholar
  4. Blanchette, E. J.: Ovarian steroid cells. I. Differentiation of the lutein cell from the granulosa follicle cell during the preovulatory stage and under the influence of exogenous gonadotrophins. J. Cell Biol. 31, 501–516 (1966).Google Scholar
  5. —: Ovarian steroid cells. II. The lutein cell. J. Cell Biol. 31, 517–542 (1966).Google Scholar
  6. Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 2. The ultrastructure of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 195–211 (1970)a.Google Scholar
  7. —: Studies of the fine structure of ovarian interstitial tissue. 3. The innervation of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 212–226 (1970b).Google Scholar
  8. —: Studies of the fine structure of ovarian interstitial tissue. 6. Effects of clomiphene on the thecal gland of the domestic fowl. Z. Zellforsch. 109, 227–244 (1970c).Google Scholar
  9. —: Studies of the fine structure of ovarian interstitial tissue. 1. A comparative study of the fine structure of the ovarian interstitial tissue in the rat and the domestic fowl. J. Anat. (Lond.) 108, 275–290 (1971a)Google Scholar
  10. —: Studies of the fine structure of ovarian interstitial tissue. 4. Effects of steroids on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 111–132 (1971b).Google Scholar
  11. —: Studies of the fine structure of ovarian interstitial tissue. 5. Effects of gonadotropins on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 133–156 (1971c).Google Scholar
  12. —: The effects of steroids on the granulosa cells in the domestic fowl. Z. Zellforsch. 119, 179–187. (1971d).Google Scholar
  13. - The effects of gonadotropins on the granulosa cells of the domestic fowl. Acta Endocrinologia (in press) (1971e).Google Scholar
  14. —: The effects of clomiphene on the granulosa cells of the domestic fowl. Z. Zellforsch. 119, 188–194 (1971f).Google Scholar
  15. Eckstein, P.: The ovary. In: Zuckerman, S. (ed.), p. 311. New York: Academic Press 1962.Google Scholar
  16. Enders, A. C.: Observations on the fine structure of lutein cells. J. Cell Biol. 12, 101–113 (1962).Google Scholar
  17. Falck, B.: Site of production of oestrogen in rat ovary as studied in microtransplants. Acta physiol. scand. 47, Suppl. 163, 1–101 (1959).Google Scholar
  18. Kjaerheim, Å.: Studies of adrenocortical ultrastructure. 1. Aldehyde perfusion fixation of the domestic fowl. Acta anat. (Basel) 74, 424–453 (1969).Google Scholar
  19. Millonig, G.: The advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol. 32, 1637 (1961).Google Scholar
  20. Mossmann, H. W., Koering, M. J., Ferry, D., Jr.: Cyclic changes of interstitial gland tissue of the human ovary. Amer. J. Anat. 115, 235–256 (1964).Google Scholar
  21. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).Google Scholar
  22. Ryan, K. J., Smith, O. W.: Biogenesis of steroid hormones in the human ovary. Recent Progr. Hormone Res. 21, 367–409 (1965).Google Scholar
  23. Ryter, A., Kellenberger, E.: L'inclusion au polyester pour l'ultramicrotomie. J. Ultrastruct. Res. 2, 200–214 (1958).Google Scholar
  24. Westman, A.: Experimentelle Studien über die funktionelle Bedeutung der Theca-internaZellen. Acta obstet, gynec. scand. 8, 290–306 (1929).Google Scholar
  25. —: Untersuchungen über die Abhängigkeit der Funktion des Corpus luteum von den Ovarialfollikeln und über die Bildungsstätte der Hormone im Ovarium. Arch. Gynäk. 158, 476–504 (1934).Google Scholar
  26. Woods, J. E., Domm, L. V.: A histochemical identification of the Androgen-producing cells in the gonads of the domestic fowl and albino rat. Gen. comp. Endocr. 7, 559–570 (1966).Google Scholar
  27. Wyburn, G. H., Baillie, A. H.: In: Horton-Smith, C., and Amoroso, E. C. (ed.), Physiology of the domestic fowl, p. 30. Edinburg: Oliver and Boyd 1966.Google Scholar
  28. Young, W. C.: The mammalian ovary. In: Sex and internal secretion, 3rd. ed., (W. C. Young, ed.), p. 449. Baltimore: Williams and Wilkins Co. 1961.Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Erik Dahl
    • 1
  1. 1.Department of Anatomy, Dental FacultyUniversity of OsloBlindernNorway

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