Molecular and General Genetics MGG

, Volume 208, Issue 1–2, pp 357–360 | Cite as

Expression of the human β-globin gene in mouse teratocarcinoma cells

  • Corinne Besnard
  • Jacques Jami
Short Communications
Received:
  • 24 Downloads

Summary

We have stably transformed PCC4 and F9 teratocarcinoma stem cells with the human β-globin gene. The transformed PCC4 cells retained their ability to produce in host mice tumours containing various differentiated tissues. Three of the five clones examined had human β-globin transcripts, with correct 5′ termini; however, some transcripts were also initiated upstream from the natural cap site in two of the three clones. The expression of the human β-globin gene was maintained when the stem cell phenotype was changed, either into endodermal cell type by retinoic acid treatment of the F9 clones, or into fibroblastic cell type by cell hybridization of transformed PCC4 cells with L fibroblasts. Thus, the human β-globin gene introduced into early embryonic cells can be expressed constitutively and its expression is maintained when the pattern of gene expression in the cells is changed.

Key words

Transfection Transcripts Endoderm Cell hybrids 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson WF, Killos L, Sanders-Haiga L, Kretschner PJ, Diacumakos EG (1980) Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts. Proc Natl Acad Sci USA 77:5399–5403Google Scholar
  2. Banerji J, Rusconi S, Schaffner W (1981) Expression of a β-globin gene is enhanced by remote SV40 DNA sequences. Cell 27:299–308Google Scholar
  3. Bensaude O, Morange M (1983) Spontaneous high expression of heat shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo. EMBO J 2:173–177Google Scholar
  4. Berk AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease digested hybrids. Cell 12:721–732Google Scholar
  5. Bernstine EG, Hooper ML, Grandchamp S, Ephrussi B (1973) Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci USA 70:3899–3903Google Scholar
  6. Besnard C, Lasserre C, Pictet R, Jami J (1984) Persistence of transcription of the human β-globin gene in hybrids between transfected F9 embryonal carcinoma and L cells. Cell Differ 15:215–219Google Scholar
  7. Brinster RL, Chen HY, Warren R, Sarthy A, Palmiter RD (1982) Regulation of metallothionein-thymidine kinase fusion plasmids injected into mouse eggs. Nature 296:39–42Google Scholar
  8. Chao MV, Mellon P, Charnay P, Maniatis T, Axel R (1983) The regulated expression of β-globin genes introduced into mouse erythroleukemia cells. Cell 32:483–493Google Scholar
  9. Charnay P, Treisman R, Mellon P, Chao M, Axel R, Maniatis T (1984) Differences in human α- and β-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences. Cell 38:251–263Google Scholar
  10. Chirgwin JM, Przybyk AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299Google Scholar
  11. Glisin V, Crkvenjakov R, Byus C (1974) Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633–2637Google Scholar
  12. Hsiung N, Warrick H, Deriel JK, Tuan D, Forget BG, Skoultchi A, Kucherlapati R (1980) Cotransfer of circular and linear prokaryotic and eukaryotic DNA sequences into mouse cells. Proc Natl Acad Sci USA 77:4852–4856Google Scholar
  13. Lawn RM, Fritsch EF, Parker RC, Blake G, Maniatis T (1978) The isolation and characterization of linked delta- and betaglobin genes from a cloned library of human DNA. Cell 15:1157–1174Google Scholar
  14. Mantei N, Boll W, Weissmann C (1979) Rabbit β-globin mRNA production in mouse L cells transformed with cloned rabbit β-globin chromosomal DNA. Nature 281:40–46Google Scholar
  15. Mintz B, Illmensee K (1975) Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci USA 72:3585–3589Google Scholar
  16. Mulligan RC, Berg P (1981) Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci USA 78:2072–2076Google Scholar
  17. Nicolas JF, Dobois P, Jakob H, Gaillard J, Jacob F (1975) Tératocarcinome de la souris: différenciation en culture d'une lignée de cellules primitives à potentialités multiples. Ann Microbiol (Paris) 126A:3–22Google Scholar
  18. Rees AR, Adamson ED, Graham CF (1979) Epidermal growth factor receptors increase during the differentiation of embryonal carcinoma cells. Nature 281:309–311Google Scholar
  19. Rosenthal A, Wright S, Cedar H, Flavell R, Grosveld F (1984) Regulated expression of an introduced MHC H-2Kbml gene in murine embryonal carcinoma cells. Nature 310:415–418Google Scholar
  20. Rousset JP, Dubois P, Lasserre C, Avilès D, Fellous M, Jami J (1979) Phenotype and surface antigens of mouse teratocarcinoma xfibroblast cell hybrids. Somatic Cell Genet 5:739–752Google Scholar
  21. Rusconi S, Schaffner W (1981) Transformation of frog embryos with a rabbit β-globin gene. Proc Natl Acad Sci USA 78:5051–5055Google Scholar
  22. Sassonne-Corsi P, Duboule D, Chambon P (1985) Viral enhancer activity in teratocarcinoma cells. Cold Spring Harbor Symp Quant Biol 50:747–752Google Scholar
  23. Scott RW, Vogt TF, Croke ME, Tilghman SM (1984) Tissue-specific activation of a cloned α-fetoprotein gene during differentiation of a transfected embryonal carcinomal cell line. Nature 310:562–567Google Scholar
  24. Solter D, Shevinsky L, Knowles BB, Strickland S (1979) The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid. Dev Biol 70:515–521Google Scholar
  25. Strickland S, Mahdavi V (1978) The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15:393–403Google Scholar
  26. Wagner EF, Mintz B (1982) Transfer of nonselectable genes into mouse teratocarinoma cells and transcription of the transferred human β-globin gene. Mol Cell Biol 2:190–198Google Scholar
  27. Wigler M, Silverstein S, Lee L-S, Pellicer A, Cheng Y, Axel R (1977) Transfer of purified Herpes Virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232Google Scholar
  28. Wold B, Wigler M, Lacy E, Maniatis T, Silverstein S, Axel R (1979) Introduction and expression of a rabbit β-globin gene in mouse fibroblasts. Proc Natl Acad Sci USA 76:5684–5688Google Scholar
  29. Wright S, de Boer E, Grosveld FG, Flavell RA (1983) Regulated expression of the human β-globin gene family in murine erythroleukemia cells. Nature 305:333–336Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Corinne Besnard
    • 1
    • 2
  • Jacques Jami
    • 1
    • 2
  1. 1.Institut Jacques Monod, Unité 257 de l'Institut National de la Santé et de la Recherche MédicaleCentre National de la Recherche Scientifique etParis Cedex 05France
  2. 2.Université Paris VIIParis Cedex 05France

Personalised recommendations