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Chromosoma

, Volume 60, Issue 1, pp 69–79 | Cite as

Comparative study of human chromosome replication in primary cultures of embryonic fibroblasts and in cultures of peripheral blood leucocytes

III. Distribution of AT- and GC-nucleotide pairs along the length of chromosomes 1, 2, 3, and 16 in the two types of human cells
  • Elena R. Lozovskaya
  • S. I. Slesinger
  • Alexandra A. Prokofieva-Belgovskaya
Article

Abstract

Distributions of AT- and GC-base pairs along the length of chromosomes 1, 2, 3 and 16 in primary cultures of embryonic fibroblasts and of peripheral blood leucocytes were studied by autoradiography with: 1. 3H-thymidine and 3H-deoxycytidine; 2. 3H-deoxyadenosine and 3H-deoxyguanosine. It has been shown that the two types of cells differ in the DNA content and proportion of AT- and GC-nucleotide pairs in the centromeric heterochromatin of chromosome I: this region contains more DNA in fibroblasts than in leucocytes mainly due to AT-pairs. In both types of cells the telomeric region of the short arm of this chromosome contains more GC- than AT-pairs. Similar results were obtained for C-heterochromatin of chromosome 16: the frequencies of labelling of this region by 3H-deoxyadenosine and 3H-thymidine in fibroblast cultures were higher than in case of 3H-deoxycytidine and 3H-deoxygyanosine, and in leucocyte cultures these frequencies were almost equal. No differences in the distributions of base pairs along the length of chromosome 2 and 3 were established in the two types of cells. — The nature of the established phenomenon may be connected with under-replication or loss in another way of part of the genetic material in the process of development and differentiation of cell systems.

Keywords

Base Pair Developmental Biology Primary Culture Genetic Material Cell System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amma, K.: Über die Differenzierung der Keimbahnzellen bei den Copepoden. Arch. Zellforsch. 6, 497–576 (1911)Google Scholar
  2. Blumenfeld, M., Forrest, H.S.: Differential underreplication of satellite DNAs during Drosophila development. Nature (Lond.) 239, 170–172 (1972)Google Scholar
  3. Boveri, T.: Die Entwicklung von Ascaris megalocephala. Festschr. f. von Kupffer 2, 383–390 (1899)Google Scholar
  4. Fox, D.P.: A non-doubling DNA series in somatic tissues of the locusts Schistocerca gregaria (Forskal) and Locusta migratoria. Chromosoma (Berl.) 29, 446–461 (1970)Google Scholar
  5. German, J., Aronian, D.: Autoradiographic studies of human chromosomes. VI. Comparison of the end of S-patterns in lymphocytes and amniotic epithelial cells. Chromosoma (Berl.) 35, 99–110 (1971)Google Scholar
  6. Gilbert, C.W., Muldal, S., Laitha, L.G., Rowley, I.: Time sequence of human chromosomes duplication. Nature (Lond.) 195, 864–873 (1962)Google Scholar
  7. Hennig, W., Meer, B.: Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) 242, 193–197 (1973)Google Scholar
  8. Jones, K.W., Corneo, G.: Location of satellite and homogeneous DNA sequences on human chromosomes. Nature (Lond.) New Biol. 233, 268 (1971)Google Scholar
  9. Kunz, W., Eckhardt, R.A.: The chromosomal distribution of satellite DNA in the germ-line and somatic tissues of the gall midge, Heteropeza pygmaea. Chromosoma (Berl.) 47, 1–19 (1974)Google Scholar
  10. Lima-de-Faria, A., Reutalu, I., Bergman, S.: The pattern of DNA synthesis in the chromosomes of man. Hereditas (Lund) 47, 695–704 (1961)Google Scholar
  11. Lozovskaya, E.R., Slesinger, S.I., Prokofieva-Belgovskaya, A.A.: Differences in the relative content of AT- and GC-nucleotide pairs in the centromeric heterochromatin of chromosome 1 in the two types of human cells. I. Molec. Biol. (USSR) 10, 937–943 (1976)Google Scholar
  12. Morishima, A., Grumbach, M., Taylor, I.: Asynchronous duplication of human chromosomes and the origin of sex chromatin. Proc. nat. Acad. Sci. (Wash.) 48, 756–763 (1962)Google Scholar
  13. Paris conference (1971): Standardization in Human Cytogenetics. Birth Defects: Original Article Series, VIII: 7, 1972Google Scholar
  14. Pearson, G.G., Timmis, I.N., Ingle, I.: The differential replication of DNA during plant development. Chromosoma (Berl.) 45, 281–294 (1974)Google Scholar
  15. Prokofieva-Belgovskaya, A.A.: Heterochromatinization as variation of chromosome cycle. Zh. Obshch. Biol., USSR 6, 93–124 (1945)Google Scholar
  16. Prokofieva-Belgovskaya, A.A., Siesinger, S.I., Lozovskaya, E.R., Atayeva, D.M.: Comparative study of human chromosome replication in primary cultures of embryonic fibroblasts and in cultures of peripheral blood leucocytes. II. Replication of centromeric regions of chromosomes at the termination of the S period. Chromosoma (Berl.) 57, 261–270 (1976)Google Scholar
  17. Rudkin, G.T., Corlette, S.L.: Disproportionate synthesis of DNA in a polytene chromosome region. Proc. nat. Acad. Sci. (Wash.) 43, 964–968 (1957)Google Scholar
  18. Siesinger, S.L., Lozovskaya, E.R., Atayeva, D.M., Prokofieva-Belgovskaya, A.A.: Comparative study of human chromosome replication in primary cultures of embryonic fibroblasts and in cultures of peripheral blood leucocytes. I. Chromosome replication at the beginning and termination of the S period. Chromosoma (Berl.) 49, 173–184 (1974b)Google Scholar
  19. Siesinger, S.L., Lozovskaya, E.R., Atayeva, D.M., Prokofieva-Belgovskaya, A.A.: A comparative study of the kinetics of termination of human chromosome replication in two cell types. Tsitologia (USSR) 16, 690–697 (1974c)Google Scholar
  20. Slesinger, S.I., Lozovskaya, E.R., Prokofieva-Belgovskaya, A.A.: Distribution of AT and GC pairs of DNA bases along chromosomes 1 and 2 in two types of human cells. Dokl. Acad. Nauk, USSR 217, 462–464 (1974a)Google Scholar
  21. Taylor, J.H.: Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J. biophys. biochem. Cytol. 7, 455–464 (1960)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Elena R. Lozovskaya
    • 1
  • S. I. Slesinger
    • 1
  • Alexandra A. Prokofieva-Belgovskaya
    • 1
  1. 1.Institute of Molecular BiologyUSSR Academy of ScienceMoscowU.S.S.R.

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