Molecular and General Genetics MGG

, Volume 199, Issue 2, pp 178–182 | Cite as

Gene transfer to cereal cells mediated by protoplast transformation

  • Horst Lörz
  • Barbara Baker
  • Jeff Schell


Direct gene transfer to cereal cells was achieved by transformation of protoplasts with naked DNA. Protoplasts isolated from cultured cells of Triticum monococcum were incubated in the presence of polyethylene glycol (PEG) with circular and linear plasmid DNA. The pBR322-derived plasmid, pBL1103-4, contained a selectable chimeric gene comprising the protein coding region of the Tn5 aminogly-coside phosphotransferase type II gene (NPT II), the nopaline synthase promoter (pNOS) and the polyadenylation signal of the octopine synthase gene. Transformed cells were selected in medium containing kanamycin and identified by detection of aminoglycoside phosphotransferase II activity.


Gene Transfer Kanamycin Polyethylene Glycol Polyadenylation Signal Protein Code Region 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Horst Lörz
    • 1
  • Barbara Baker
    • 1
  • Jeff Schell
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKöln 30Federal Republic of Germany

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