Archives of Toxicology

, Volume 32, Issue 4, pp 347–350 | Cite as

Effect of temperature on the activity of human blood cholinesterases

  • E. Reiner
  • A. Buntić
  • M. Trdak
  • V. Simeon
Original Investigations
Received:

Abstract

Temperature conversion tables for activities of human whole blood and plasma cholinesterases were worked out for the temperature range 10° to 40°C. The tables are intended to be used in routine measurement of cholinesterase activity. Enzyme activity was measured by the method of Ellman et al. (1961), which is widely used for screening exposure to anticholinesterase compounds.

Key words

Human Blood Cholinesterases Temperature and Cholinesterases Temperature Conversion Table 

Zusammenfassung

Temperaturumrechnungstabellen für die Aktivität der Cholinesterasen im menschlichen Blut und Plasma wurden für den Temperaturenbereich von 10° bis 40 °C ausgefertigt. Die Tabellen sind für Routinemessungen der Cholinesterasenaktivität bestimmt. Zur Messung der Aktivität wurde die Methode von Ellman et al. (1961) benutzt; diese Methode hat große Anwendung gefunden bei Kontrollen der Exposition von Cholinesterasehemmstoffen.

Schlüsselwörter

Cholinesterasen im menschlichen Blut und Plasma Temperatur und Cholinesterasen Temperaturumrechnungstabellen 
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References

  1. Augustinsson, K. B.: Determination of activity of cholinesterases. In: Analysis of biogenic amines and their related enzymes, Series: Methods in biochemical analysis (Glick, D., Ed.), pp. 217–273. New York: Wiley, Interscience Publ. 1971Google Scholar
  2. Bates, R. G.: Determination of pH, theory and practice, p. 76. New York: Wiley, Interscience Publ. 1964Google Scholar
  3. Edson, E. F.: Blood tests for users of O.P. insecticides. Wld Crops 10, 49 (1958); and Instruction manual from Tintometric Ltd. Salisbury, England; The rapid field determination of cholinesterase, Simplification of field procedureGoogle Scholar
  4. Ellman, G. L., Courtney, K. D., Andres, V., Featherstone, R. M.: A new and rapid colorimetric determination of acethylcholinesterase activity. Biochem. Pharmacol. 7, 88–95 (1961)Google Scholar
  5. Garry, Ph. J., Routh, J. I.: A micro method for serum cholinesterase. Clin. Chem. 11, 91–96 (1965)Google Scholar
  6. Oudart, J. L., Holmstedt, B.: Determination of plasma cholinesterase activity by means of a test paper and its use in the field. Arch. Toxicol. 27, 1–12 (1970)Google Scholar
  7. Pleština, R.: Our experience in applying the Acholest method for determination of human plasma cholinesterase activity (in Croatian). Arh. Hig. Rada 17, 291–301 (1966)Google Scholar
  8. Pleština, R., Davis, A., Bailey, D. R.: Effect of metrifonate on blood cholinesterases in children during the treatment of schistosomiasis. Bull. Wld Hlth Org. 46, 747–759 (1972)Google Scholar
  9. Richterich, R.: Bestimmung der Serumcholinesterase mit Hilfe eines Indikatorpapiers. Schweiz. med. Wschr. 92, 263–265 (1962)Google Scholar
  10. Vandekar, M., Hedayat, S., Pleština, R., Ahmady, G.: A study of the safety of O-isopropoxyphenylcarbamate in an operational field-trial in Iran. Bull. Wld Hlth Org. 38, 609–623 (1968)Google Scholar
  11. Wilhelm, K.: Determination of human plasma cholinesterase activity by adapted Ellman's method. Arh. Hig. Rada 19, 199–207 (1968)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • E. Reiner
    • 1
  • A. Buntić
    • 1
  • M. Trdak
    • 1
  • V. Simeon
    • 1
  1. 1.Yugoslav Academy of Sciences and ArtsInstitute for Medical ResearchZagrebCroatia, Yugoslavia

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