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Archiv für Toxikologie

, Volume 28, Issue 4, pp 279–285 | Cite as

Adenosine deaminase and protein pattern in serum and cerebrospinal fluid in experimental manganese encephalopathy

  • S. J. Mustafa
  • Satya V. Chandra
Original Investigations

Abstract

Chronic manganese toxicity was produced in rabbits by intra-tracheal administration of manganese dioxide (400 mg); resulting in hind limb paralysis. The animals were sacrificed at a period of 24 months. Adenosine deaminase activity was determined in cerebrospinal fluid (CSF), whole brain and serum, whereas protein patterns were studied only in serum and CSF. The total serum proteins increased by 23% due to increase in globulins. In CSF there was marked reduction in the concentration of albumin and globulins, thus, resulting in significant lowering of total proteins. Further our findings of marked elevation in adenosine deaminase only in CSP of experimental animals suggests that this test may be used as a clinical diagnostic index in patients with chronic manganese poisoning.

Key words

Brain Diseases Manganese Chronic Intoxication 

Zusammenfassung

Einmalige intratracheale Verabfolgung von Mangandioxid (400 mg) an Kaninchen erzeugte chronische Manganvergiftung mit Lähmung der Hinterextremitäten. 24 Monate später werden die Tiere getötet und die Adenosindeaminase-Aktivität wird in Liquor, Gesamtgehirn und Serum, das Proteinmuster in Serum und Liquor bestimmt. Die Gesamtproteine im Serum sind um 23 % erhöht infolge Globulinvermehrung. Im Liquor ist die Albumin- und Globulin-Konzentration deutlich vermindert bei erniedrigtem Gesamtprotein. Unser Befund einer deutlichen Erhöhung der Adenosindeaminase-Aktivität allein im Liquor deutet an, daß dieser Test als klinisch-diagnostische Probe bei Patienten mit chronischer Manganvergiftung eingesetzt werden könnte.

Schlüsselwörter

Hirnerkrankungen Mangan Chronische Vergiftung 

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References

  1. Canavan, M.M., Cobb, S., Drinker, C.K.: Chronic manganese toxicity. Arch. Neurol. Psychiat. 32, 501–512 (1934).Google Scholar
  2. Chandra, S.V., Srivastava, S.P.: Experimental production of early brain lesions in rats by parenteral administration of manganese chloride. Acta pharmacol. (Kbh.) 38, 177–183 (1970).Google Scholar
  3. Cotzias, G.C., Foradori, A.C.: Trace metal metabolism, The biological basis of medicine (6Bittar, E.E. and N. Bittar, eds.), vol. 1, p. 105–121. New York: A P 1968.Google Scholar
  4. Hassanein, M., Ghaleb, H.A., Haroun, E.A., Hegazy, M.R., Khayyal, M.A.H.: Chronic manganism: Preliminary observation on glucose tolerance and serum proteins. Brit. J. industr. Med. 23, 67–70 (1966).Google Scholar
  5. Holmberg, C. G., Laurell, C.B.: Investigations in serum Copper III. Isolation of the Copper containing protein and a description of some of its properties. Acta chem. scand. 2, 550–556 (1948).Google Scholar
  6. Letnansky, K., Seelich, F.: Activity of adenosine deaminase in cancer with particular emphasis on bronchial carcinoma. Klin. Wschr. 36, 826–828 (1958).Google Scholar
  7. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin-Phenol reagent. J. biol. Chem. 195, 265–275 (1951).Google Scholar
  8. Mena, I., Marin, O., Fuenzahidd, S., Cotzias, G.C.: Chronic manganese poisoning, clinical picture and manganese turnover. Neurology (Minneap.) 17, 128–136 (1967).Google Scholar
  9. Mustafa, S.J., Chandra, S.V.: Levels of 5-hydroxytryptamine, dopamine and norepinephrine in whole brain of rabbits in chronic manganese toxicity. J. Neurochem. 18, 931–933 (1971).Google Scholar
  10. — Tewari, C.P.: Latent adenosine deaminase in mouse brain. I. Exposure and solubilization of the mitochondrial enzyme. Biochim. biophys. Acta (Amst.) 198, 93–100 (1970).Google Scholar
  11. Raczynska, J., Jonas, S., Krawczynski, J.: Diagnostic value of adenosine deaminase in some liver diseases. Clin. chim. Acta 13, 151–154 (1966).Google Scholar
  12. Sassowa, J.: Adenosine deaminase in serum of children. I. Adenosine deaminase activity in serum of normal children and in certain diseases. Pol. Tyg. lek. 17, 1408–1412 (1962).Google Scholar
  13. Secchi, G.C., Rezzonica, A., Gervasini, N.: Adenosine deaminase activity in liver diseases. Enzym. biol. clin. 8, 67–72 (1967).Google Scholar
  14. Straub, F.B., Stephaneck, O., Acs, G.: The adenosine deaminase activity of plasma of cancer patients. Biokhimiya 22, 118–121 (1957).Google Scholar
  15. Zaidi, S.H.: Experimental pneumoconiosis, p. 33. Baltimore: John Hopkins 1969.Google Scholar
  16. Zotti, C., Ipata, P.L.: Adenosine deaminase activity of the cerebrospinal fluid and its diagnostic value in subjects affected with tumours of the central nervous system. Riv. Neurobiol. 7, 481–488 (1961).Google Scholar
  17. Zotti, G.: Presence of adenosine deaminase activity in cerebrospinal fluid. Boll. Soc. ital. Biol. sper. 37, 1073–1074 (1961).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • S. J. Mustafa
    • 1
  • Satya V. Chandra
    • 1
  1. 1.Industrial Toxicology Research CentreLucknow, U. P.India

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