Archiv für Toxikologie

, Volume 28, Issue 4, pp 279–285 | Cite as

Adenosine deaminase and protein pattern in serum and cerebrospinal fluid in experimental manganese encephalopathy

  • S. J. Mustafa
  • Satya V. Chandra
Original Investigations


Chronic manganese toxicity was produced in rabbits by intra-tracheal administration of manganese dioxide (400 mg); resulting in hind limb paralysis. The animals were sacrificed at a period of 24 months. Adenosine deaminase activity was determined in cerebrospinal fluid (CSF), whole brain and serum, whereas protein patterns were studied only in serum and CSF. The total serum proteins increased by 23% due to increase in globulins. In CSF there was marked reduction in the concentration of albumin and globulins, thus, resulting in significant lowering of total proteins. Further our findings of marked elevation in adenosine deaminase only in CSP of experimental animals suggests that this test may be used as a clinical diagnostic index in patients with chronic manganese poisoning.

Key words

Brain Diseases Manganese Chronic Intoxication 


Einmalige intratracheale Verabfolgung von Mangandioxid (400 mg) an Kaninchen erzeugte chronische Manganvergiftung mit Lähmung der Hinterextremitäten. 24 Monate später werden die Tiere getötet und die Adenosindeaminase-Aktivität wird in Liquor, Gesamtgehirn und Serum, das Proteinmuster in Serum und Liquor bestimmt. Die Gesamtproteine im Serum sind um 23 % erhöht infolge Globulinvermehrung. Im Liquor ist die Albumin- und Globulin-Konzentration deutlich vermindert bei erniedrigtem Gesamtprotein. Unser Befund einer deutlichen Erhöhung der Adenosindeaminase-Aktivität allein im Liquor deutet an, daß dieser Test als klinisch-diagnostische Probe bei Patienten mit chronischer Manganvergiftung eingesetzt werden könnte.


Hirnerkrankungen Mangan Chronische Vergiftung 


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • S. J. Mustafa
    • 1
  • Satya V. Chandra
    • 1
  1. 1.Industrial Toxicology Research CentreLucknow, U. P.India

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