Molecular and General Genetics MGG

, Volume 197, Issue 3, pp 522–524 | Cite as

Plasmid transformation in Bacillus subtilis: Factors affecting the synapsis of donor and recipient DNA

  • Piotr Ceglowski
  • Thomas A. Trautner
Short Communication
Received:
  • 27 Downloads

Summary

Hybrid plasmids were constructed in which the transcription of regions of inserted DNA was defined. Cells containing these plasmids were transformed with monomeric forms of a different hybrid plasmid, which contained, however, the same inserted DNA as the resident plasmid. The transformation frequencies observed indicated that transcription of homologous DNA in resident plasmids and also tertiary DNA structure interfered with transformation.

Keywords

Bacillus Bacillus Subtilis Monomeric Form Transformation Frequency Hybrid Plasmid 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Piotr Ceglowski
    • 1
  • Thomas A. Trautner
    • 1
  1. 1.Max-Planck-Institut für molekulare GenetikBerlin 33

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