Advertisement

Molecular and General Genetics MGG

, Volume 209, Issue 1, pp 15–20 | Cite as

Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins

  • Gregory A. Bohach
  • Patrick M. Schlievert
Article

Summary

The nucleotide sequence for the structural gene entC1 encoding staphylococcal enterotoxin C1 was determined. The gene contained 801 bp and coded for a protein of 266 amino acids. Of these, 27 comprised the signal peptide. Cleavage of the signal peptide resulted in a mature protein with 239 amino acids and a calculated molecular weight of 27496. The nucleotide sequence of entC1 shared considerable homology (74% and 59%, respectively) with genes encoding enterotoxin B and streptococcal pyrogenic exotoxin A. A similar degree of amino acid homology was observed after alignment of the respective proteins. Thus, certain regions of these three toxin molecules possess structural similarities that may be responsible for shared biological properties.

Key words

Staphylococcus aureus Streptococcus pyogenes Enterotoxin C1 Pyrogenic toxins Homology 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altboum Z, Hertman I, Sarid S (1985) Penicillinase plasmid-linked genetic determinants for enterotoxins B and C1 production in Staphylococcus aureus. Infect Immun 47:514–521Google Scholar
  2. Bergdoll MS, Robbins RN, Weiss K, Borja CR, Huang IY, Chu FS (1973) The staphylococcal enterotoxins: similarities. In: Jeljaszewicz J, Hryniewicz W (eds) Contributions to microbiology and immunology, vol 1. S. Karger, Basel, p 390Google Scholar
  3. Betley MJ, Bergdoll MS (1981) Staphylococcal enterotoxin type C genes not associated with extrachromosomal DNA. Abstracts of the annual meeting of the American Society for Microbiology, ASM, Washington DC D38, p 49Google Scholar
  4. Betley MJ, Mekalanos JM (1985) Staphylococcal enterotoxin A is encoded by phage. Science 229:185–187Google Scholar
  5. Blomster-Hautamaa DB, Kreiswirth BN, Kornblum JS, Novick RP, Schlievert PM (1986) The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1. J Biol Chem 261:15783–15786Google Scholar
  6. Bohach GA, Schlievert PM (1987) Expression of staphyococcal enterotoxin C1 in Escherichia coli. Infect Immun 55:428–432Google Scholar
  7. Brunson KW, Watson DW (1974) Pyrogenic specificity of streptococcal exotoxins, staphylococcal enterotoxin and gram-negative endotoxin. Infect Immun 10:347–351Google Scholar
  8. Dolman CE, Wilson RJ (1938) Experiments with staphylococcal enterotoxin. J Immunol 35:13–30Google Scholar
  9. Hewick RM, Hunkapiller MW, Hood LE, Dreyer WJ (1981) A gas-liquid solid phase peptide and protein sequenator. J Biol Chem 256:7990–7997Google Scholar
  10. Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197Google Scholar
  11. Huang IY, Bergdoll MS (1970) The primary structure of staphylococcal enterotoxin B. III. The cyanogen bromide peptides of reduced and amino ethylated enterotoxin B, and the complete amino acid sequence. J Biol Chem 245:3518–3525Google Scholar
  12. Johnson LP, Schlievert PM (1984) Group A streptococcal phage T12 carries the structural gene for pyrogenic exotoxin type A. Mol Gen Genet 194:52–56Google Scholar
  13. Johnson LP, Schlievert PM, Watson DW (1980) Transfer of group A streptococcal pyrogenic exotoxin production to nontoxigenic strains by lysogenic conversion. Infect Immun 28:254–257Google Scholar
  14. Johnson LP, L'Italien JJ, Schlievert PM (1986) Streptococcal pyrogenic exotoxin type A (scarlet fever toxin) is related to Staphylococcus aureus enterotoxin B. Mol Gen Genet 203:354–356Google Scholar
  15. Jones CJ, Khan SA (1986) Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus. J Bacteriol 166:29–33Google Scholar
  16. Kreiswirth BN, Lofdahl S, Betley MJ, O'Reilly M, Schlievert PM, Bergdoll MS, Novick RP (1983) The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709–712Google Scholar
  17. Kushner SR (1978) An improved method for transformation of Escherichia coli with ColE1 derived plasmids. In: Boyer HB, Nicosia S (eds) Genetic engineering. Elsevier/North-Holland, Amsterdam, p 17Google Scholar
  18. Mclaughlin JR, Murray CL, Rabinowitz JC (1981) Unique features in the ribosome binding site sequence of the gram-positive of Staphylococcus aureus β-lactamase gene. J Biol Chem 256:11283–11291Google Scholar
  19. Messing J (1979) A multi-purpose cloning system based on the single-stranded DNA bacteriophage M13. Recombinant DNA Technical Bulletin, NIH Publication No 79–99, 2: No. 2, 43–48Google Scholar
  20. Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78Google Scholar
  21. Poindexter NJ, Schlievert PM (1985) The biochemical and immunological properties of toxic-shock syndrome toxin-1 (TSST-1) and association with TSS. J Toxicol Toxin Rev 4:1–39Google Scholar
  22. Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353Google Scholar
  23. Sako T, Tsuchida N (1983) Nucleotide sequence of the staphylokinase gene from Staphylococcus aureus. Nucleic Acids Res 11:7679–7693Google Scholar
  24. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  25. Schlievert PM, Schoettle DJ, Watson DW (1979) Purification and physicochemical and biochemical characterization of a staphylococcal pyrogenic exotoxin. Infect Immun 23:609–617Google Scholar
  26. Schmidt JJ, Spero L (1983) The complete amino acid sequence of staphylococcal enterotoxin C1. J Biol Chem 258:6300–6306Google Scholar
  27. Schwab JH, Watson DW, Cromartie WJ (1955) Further studies of group A streptococcal factors with lethal and cardiotoxic properties. J Infect Dis 96:14–18Google Scholar
  28. Shine J, Dalgarno L (1974) The 3′-terminal sequence of E. coli 16S ribosomal RNA: Complementarity to nonsence triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346Google Scholar
  29. Smith BG, Johnson HM (1975) The effect of staphylococcal enterotoxins on the primary in vitro immune response. J Immunol 115:575–578Google Scholar
  30. Sugiyama H, McKissic EM, Bergdoll MS, Heller B (1964) Enhancement of bacterial endotoxin lethality by staphylococcal enterotoxin. J Infect Dis 114:111–118Google Scholar
  31. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268Google Scholar
  32. Watson DW (1960) Host-parasite factors in group A streptococcal infections. Pyrogenic and other effects of immunologic distinct exotoxins related to scarlet fever toxins. J Exp Med 111:255–284Google Scholar
  33. Weeks CR, Ferretti JJ (1986) Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12. Infect Immun 52:144–150Google Scholar
  34. Wilbur WJ, Lipman DJ (1983) Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci USA 80:726–730Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Gregory A. Bohach
    • 1
  • Patrick M. Schlievert
    • 1
  1. 1.Department of MicrobiologyUniversity of Minnesota Medical SchoolMinneapolisUSA

Personalised recommendations