Molecular and General Genetics MGG

, Volume 209, Issue 2, pp 408–410 | Cite as

Cloning and sequencing of the HU-2 gene of Escherichia coli

  • Yasunobu Kano
  • Katsuaki Osato
  • Morimasa Wada
  • Fumio Imamoto
Short Communications


The Escherichia coli HU-2 gene was cloned using a DNA fragment from the HU-1 gene as a probe. The amino acid sequence of the HU-2 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream of the translation initiation codon (AUG) and a possible rhoindependent terminater site downstream of the termination codon (UAA) of the gene.

Key words

Escherichia coli HU-2 protein Gene cloning Nucleotide sequence 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Yasunobu Kano
    • 1
  • Katsuaki Osato
    • 2
  • Morimasa Wada
    • 1
  • Fumio Imamoto
    • 1
  1. 1.Laboratory of Molecular Genetics, (Riken) Tsukuba Life Science CenterThe Institute of Physical and Chemical ResearchTsukuba, IbarakiJapan
  2. 2.Biotechnology Research Department, Research and Development DivisionJGC corporationYokohamaJapan

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