Molecular genetic analysis of the pyr-4 gene of Neurospora crassa
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By means of S1 nuclease mapping, one transcription origin and three termini are identified for the pyr-4 gene of Neurospora crassa, the same origin being used also by Escherichia coli on the cloned gene. Translation of the clone in mini-cells gives a 50000 dalton gene product, the same size as that determined for the Neurospora native enzyme. Putative CAAT and TATA boxes, and upstream and downstream potential secondary structures, are identified.
Key wordsOrotidine 5′-monophosphate decarboxylase Promoter CAAT box TATA box Minicells
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