The human endothelial cell in tissue culture

  • Yuji Maruyama


Endothelial cells of the human umbilical vein were isolated by trypsin and cultured. Histological preparations of those umbilical cords were made to check the removal of the endothelium and not of other tissues.

The cells in culture grew in sheets which were identified with endothelial cells. Isolated cells rarely showed a spindle shape. Further investigation is needed to distinguish spindle-shaped cells from fibroblasts or other cellular elements.

Identification of endothelial cells in the previous reports and materials used are discussed.


Endothelial Cell Tissue Culture Trypsin Umbilical Cord Umbilical Vein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Altschul, R.: Endothelium, its development, morphology, function, and pathology. New York: Macmillan 1954.Google Scholar
  2. Benewolenskaja, S. W.: Hematopoiesis in cultures of embryonic liver of man. Arch. exp. Zellforsch. 9, 128–141 (1930).Google Scholar
  3. Bornstein, M. B.: Reconstituted rat-tail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes. Lab. Invest. 7, 134–137 (1958).Google Scholar
  4. Cameron, G., and R. Chambers: Neoplasm studies III. Organization of cells of human tumors in tissue culture. Amer. J. Cancer 30, 115–129 (1937).Google Scholar
  5. Fujita, T.: Histological studies on the neuro-insular complex in the pancreas of some mammals. Z. Zellforsch. 50, 94–109 (1959).Google Scholar
  6. Gomori, G.: Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. exp. Biol. Med. (N. Y.) 42, 23–26 (1939).Google Scholar
  7. —: The distribution of phosphatase in normal organs and tissues. J. cell. comp. Physiol. 17, 71–83 (1941).Google Scholar
  8. Herzog, G., u. W. Schopper: Über das Verhalten der Blutgefäße in der Kultur. Arch. exp. Zellforsch. 11, 202–218 (1931).Google Scholar
  9. Honjin, R., T. Nakamura, Y. Okayama, Z. Nunogami and T. Wazima: Histochemistry of alkaline phosphatase. Report 2. A new histochemical procedure for alkaline phosphatase with alizarin red. Acta Anat. Nippon 37, 126–133 (1962).Google Scholar
  10. Hueper, W. C., and M. A. Russell: “Capillary-like formations” in tissue cultures of leucocytes. Arch. exp. Zellforsch. 12, 407–424 (1932).Google Scholar
  11. Kabat, E. A., and J. Furth: A histochemical study of the distribution of alkaline phosphatase in various normal and neoplastic tissues. Amer. J. Path. 17, 303–318 (1941).Google Scholar
  12. Khlopin, N. G.: Changes in the endothelium of the arteries, veins and heart in experimental conditions. Voprosy Etiologii I Pathogeneza Opukholei (Leningrad) 1957, 21–25 (Abst.). Excerpta med. (Amst.), Sect. I, 14, 425 (1960).Google Scholar
  13. —, and N. M. Chistova: Endothelial tissue culture. Doklady Biol. Sci. Sect. 119, 217–220 (1958) (Abst.). Excerpta med. (Amst.), Sect. I, 14, 596 (1960).Google Scholar
  14. Landow, H., E. A. Kabat and W. Newman: Distribution of alkaline phosphatase in normal and in neoplastic tissues of the nervous system, a histochemical study. Arch. Neurol. Psychiat. (Chic.) 48, 518–530 (1942).Google Scholar
  15. Lewis, W. H.: Smooth muscle and endothelium in tissue culture. Anat. Rec. 21, 72 (1921).Google Scholar
  16. —: Endothelium in tissue culture. Amer. J. Anat. 30, 39–59 (1922).Google Scholar
  17. —: The outgrowth of endothelium and capillaries in tissue culture. Bull. Johns Hopk. Hosp. 48, 242–253 (1931).Google Scholar
  18. Maximow, A. A.: Behavior of endothelium of blood vessels in tissue culture. Anat. Rec. 29, 369 (1925).Google Scholar
  19. Murray, M. R., and A. P. Stout: Cultural characteristics of a hemangioendothelioma. Amer. J. Path. 20, 277–283 (1944).Google Scholar
  20. Nishibe, M.: Growth of endocardial cells from the chick embryo heart in vitro. Arch. exp. Zellforsch. 7, 333–343 (1928–1929).Google Scholar
  21. Parker, R. C.: The development of organized vessels in cultures of blood cells. Science 77, 544–546 (1933).Google Scholar
  22. Rienhoff jr., W. F.: Development and growth of the metanephros or permanent kidney in chick embryos (8 to 10 days incubation). Bull. Johns Hopk. Hosp. 33, 392–406 (1922).Google Scholar
  23. Schopper, W.: Netzexplantation. Verh. dtsch. path. Ges. 24, 25–28 (1929).Google Scholar
  24. Scriba, K.: Explantationsstudien über das Gefäßwachstum bei 9 Tage alten Hühnerembryonen. Arch. exp. Zellforsch. 17, 68–77 (1935).Google Scholar
  25. Silberberg, M.: Endothel in der Gewebskultur. Arch. exp. Zellforsch. 9, 36–53 (1930).Google Scholar
  26. Takamatsu, H.: Histologische und biochemische Studien über die Phosphatase. I. Histochemische Untersuchungsmethodik der Phosphatase und deren Verteilung in verschiedenen Organen und Geweben. Trans. Soc. Path. Jap. 29, 492–498 (1939).Google Scholar
  27. Törö, E.: Untersuchungen über die Potenz der Endothelzellen bei der Gefäßbildung in der Gewebekultur. Arch. exp. Zellforsch. 20, 156–171 (1937).Google Scholar
  28. White, J. F., and M. S. Parshley: Growth in vitro of blood vessels from bone marrow of adult chicken. Amer. J. Anat. 89, 321–345 (1951).Google Scholar

Copyright information

© Springer-Verlag 1963

Authors and Affiliations

  • Yuji Maruyama
    • 1
  1. 1.Department of SurgeryTokyo University School of MedicineTokyo

Personalised recommendations