, Volume 105, Issue 1, pp 94–99 | Cite as

Long-term persistence in a changing climate: DNA analysis suggests very old ages of clones of alpine Carex curvula

  • Thomas Steinger
  • Christian Körner
  • Bernhard Schmid
Population Ecology Original Paper


Carex curvula is a very slow-growing rhizomatous sedge that forms extensive stands in the European an alpine belt. The recruitment of sexual progeny is extremely rare and propagation occurs predominantly through clonal growth. The randomly amplified polymorphic DNA (RAPD) technique was used to analyse clonal structure in a small patch (2.0x0.4 m sampling transect plus some additional samples) of a high-alpine population of the species. Amplification of the DNA of 116 tiller samples from the patch with eight ten-base primers yielded a total of 95 bands, of which 73 were polymorphic. Based on the RAPD amplification profiles a total of 15 multilocus genotypes (putative clones) were identified. Due to the high number of polymorphic loci the number of genetic markers delineating individual clones was high (range: 16–39 markers) which suggests that our estimates of clonal diversity are precise. More than half of the sampled tillers were identified as belonging to a single clone which formed a relatively homogeneous disc intermingling with other clones only at its margin. Based on the maximum diameter of this large clone of more than 7000 tillers and estimates of annual expansion growth of rhizomes (0.4 mm year-1), the age of the clone was calculated to be around 2000 years. This demonstrates that clones of C. curvula may persist on a single spot over long periods with quite diverse alpine climates ranging from rather mild periods in the Middle Ages to cool periods during the so called “little ice age” in the last century. Our results suggest caution with plant migration scenarios based on shifting isotherms where late-successional clonal species, which dominate the alpine vegetation all over the world, are concerned.

Key words

Alpine grassland Climate change RAPD Population genetic structure Tussock sedge 


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  1. Abrahamson WG (1980) Demography and vegetative reproduction. In: Solbrig OT (ed) Demography and evolution in plant populations. Blackwell, Oxford, pp 89–106Google Scholar
  2. Cenis JL, Perez P, Fereres A (1993) Identification of aphid (Homoptera: Aphididae) species and clones by random amplified polymorphic DNA. Ann Entomol Soc Am 86:545–550Google Scholar
  3. Cook RE (1983) Clonal plant populations. Am Sci 71:244–253Google Scholar
  4. Ellenberg H (1978) Vègetation Mitteleuropas mit den Alpen in ökologischer Sicht. Eugen Ulmer, StuttgartGoogle Scholar
  5. Ellstrand NC, Roose ML (1987) Patterns of genotypic diversity in clonal plant species. Am J Bot 74:123–131Google Scholar
  6. Eriksson O (1989) Seedling dynamics and life-histories in clonal plants. Oikos 55:231–238Google Scholar
  7. Eriksson O, Bremer B (1993) Genet dynamics of the clonal plant Rubus saxatilis. J Ecol 81:533–542Google Scholar
  8. Fager EW (1972) Diversity: a sampling study. Am Nat 106:293–310Google Scholar
  9. Grabherr G, Mähr E, Reisigl H (1978) Nettoprimärproduktion und Reproduktion in einem Krummseggenrasen (Caricetum curvulae) der Ötztaler Alpen, Tirol. Oecol Plant 13:227–251Google Scholar
  10. Handel SN (1985) The intrusion of clonal growth patterns on plant breeding systems. Am Nat 125:367–384Google Scholar
  11. Hess HE, Landolt E, Hirzel R (1967) Flora der Schweiz, vol 1. Birkhäuser, BaselGoogle Scholar
  12. Hsiao JY, Rieseberg LH (1994) Population genetic structure of Yushania niitakayamensis (Bambusoideae, Poaceae) in Taiwan. Mol Ecol 3:201–208Google Scholar
  13. Kemperman JA, Barnes BV (1976) Clone size in American aspens. Can J Bot 54:2603–2607Google Scholar
  14. Körner C, Diemer M, Schäppi B, Zimmermann L (1995) The response of alpine vegetation to elevated CO2. In: Koch GW, Mooney HA (eds) Terrestrial ecosystem response to elevated CO2 (Physiological ecology series). Academic Press, San Diego, in pressGoogle Scholar
  15. Lynch M (1990) The similarity index and DNA fingerprinting. Mol Biol Evol 7:478–484Google Scholar
  16. McClintock KA, Waterway MJ (1993) Patterns of allozyme variation and clonal diversity in Carex lasiocarpa and C. pellita (Cyperaceae). Am J Bot 80:1251–1263Google Scholar
  17. Mogie M, Hutchings MJ (1990) Phylogeny, ontogeny and clonal growth in vascular plants. In: Groenendael J van, Kroon H de (eds) Clonal growth in plants. SPD Academic, The HagueGoogle Scholar
  18. Oinonen (1967) The correlation between the size of Finnish bracken (Pteridium aquilinum (L.) Kuhn) clones and certain periods of site history. Acta For Fenn 83:1–51Google Scholar
  19. Okamura B, Jones CS, Noble LR (1993) Randomly amplified polymorphic DNA analysis of clonal population structure and geographic variation in a freshwater bryozoan. Proc R Soc Lond B 253:147–154Google Scholar
  20. Pielou EC (1969) An introduction to mathematical ecology. Wiley, New YorkGoogle Scholar
  21. Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues (Plant molecular biology manual). Kluwer, BelgiumGoogle Scholar
  22. Schäppi B, Körner Ch (1995) Growth responses of an alpine grassland to elevated CO2. Oecologia (in press)Google Scholar
  23. Schmid B (1986) Spatial dynamics and integration within clones of grassland perennials with different growth form. Proc R Soc Lond B, 228:173–186Google Scholar
  24. Schmid B (1990) Some ecological and evolutionary consequences of modular organization and clonal growth in plants. Evol Trends Plants 4:25–34Google Scholar
  25. Schmid B, Bazzaz FA (1992) Growth responses of rhizomatous plants to fertilizer application and interference. Oikos 65:13–24Google Scholar
  26. Silvertown JW, Lovett Doust J (1993) Introduction to plant population biology. Blackwell, OxfordGoogle Scholar
  27. Smith ML, Bruhn JN, Anderson JB (1992) The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature 356:428–431Google Scholar
  28. Soane ID, Watkinson AR (1979) Clonal variation in populations of Ranunculus repens. New Phytol 82:557–573Google Scholar
  29. Watkinson AR, Powell JC (1993) Seedling recruitment and the maintenance of clonal diversity in plant populations — a computer simulation of Ranunculus repens. J Ecol 81:707–717Google Scholar
  30. Waterway MJ (1994) Clonal identification and clonal diversity in Carex section Limosae in Canadian mires (Poster abstract 2046). In: Botanical Congress Yokohama, JapanGoogle Scholar
  31. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Thomas Steinger
    • 1
  • Christian Körner
    • 1
  • Bernhard Schmid
    • 1
  1. 1.Botanical InstituteUniversity of BaselBaselSwitzerland

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