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Molecular and General Genetics MGG

, Volume 198, Issue 1, pp 84–89 | Cite as

Functional characterization of the prokaryotic mobile genetic element IS26

  • Shigeru Iida
  • Beat Mollet
  • Jürg Meyer
  • Werner Arber
Article

Summary

IS26L and IS26R are the 820 bp long elements found as direct repeats at both ends of the kanamycin resistance transposon Tn2680. They can mediate cointegration in E. coli K12 which contains no IS26 in its chromosome. Cointegration occurs in rec+ or recA- strains with similar frequency. Upon cointegration mediated by either IS26R or IS26L, the element is duplicated and integrated into one of many different sites. Both IS26L and IS26R carry 14 bp perfect terminal inverted repeats and generate 8 bp direct repeats at their target sequences. Deletion formation mediated by IS26R was also observed. These functional and structural features of IS26 are characteristic of a prokaryotic mobile genetic element.

Keywords

Structural Feature Kanamycin Target Sequence Inverted Repeat Functional Characterization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Shigeru Iida
    • 1
  • Beat Mollet
    • 1
  • Jürg Meyer
    • 1
  • Werner Arber
    • 1
  1. 1.Department of Microbiology, BiozentrumUniversity of BaselBaselSwitzerland

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