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Oecologia

, Volume 107, Issue 3, pp 386–394 | Cite as

15N natural abundances and N use by tundra plants

  • K. Nadelhoffer
  • G. Shaver
  • B. Fry
  • A. Giblin
  • L. Johnson
  • R. McKane
Ecosystems Ecology

Abstract

Plant species collected from tundra ecosystems located along a north-south transect from central Alaska to the north coast of Alaska showed large and consistent differences in 15N natural abundances. Foliar δ15N values varied by about 10% among species within each of two moist tussock tundra sites. Differences in 15N contents among species or plant groups were consistent across moist tussock tundra at several other sites and across five other tundra types at a single site. Ericaceous species had the lowest δ15N values, ranging between about −8 to −6‰. Foliar 15N contents increased progressively in birch, willows and sedges to maximum δ15N values of about +2‰ in sedges. Soil 15N contents in tundra ecosystems at our two most intensively studied sites increased with depth and δ15N values were usually higher for soils than for plants. Isotopic fractionations during soil N transformations and possibly during plant N uptake could lead to observed differences in 15N contents among plant species and between plants and soils. Patterns of variation in 15N content among species indicate that tundra plants acquire nitrogen in extremely nutrient-poor environments by competitive partitioning of the overall N pool. Differences in plant N sources, rooting depth, mycorrhizal associations, forms of N taken up, and other factors controlling plant N uptake are possible causes of variations in δ15N values of tundra plant species.

Key words

Arctic 15N abundance N cycle Nitrate reductase Tundra 

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References

  1. Abuzinadah RH, Read DJ (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants. I. Utilization of peptides and proteins by ectomycorrhizal fungi. New Phytol 103: 481–493Google Scholar
  2. Al Gharbi A, Hipkin CR (1984) Studies on nitrate reductase activity in British angiosperms. I. A comparison of nitrate reductase activity in ruderal, woodland-edge and woody species. New Phytol 97: 629–639Google Scholar
  3. Armstrong RA, McGehee R (1980) Competitive exclusion. Am Nat 115: 151–170Google Scholar
  4. Atkin OK, Villar R, Cummins W (1993) The ability of several high arctic plant species to utilize nitrate nitrogen under field conditions. Oecologia 96: 239–245Google Scholar
  5. Berendse F (1979) Competition between plant populations with different rooting depths. I. Theoretical considerations. Oecologia 43: 19–26Google Scholar
  6. Chapin FS III, Fetcher N, Kielland K, Everett KR, Linkins AE (1988) Productivity and nutrient cycling of Alaskan tundra enhanced by flowing soil water. Ecology 69: 693–702Google Scholar
  7. Chapin FS III, Moilanen L, Kielland K (1993) Preferential use of organic nitrogen by a non-mycorrhizal arctic sedge. Nature 361: 150–153Google Scholar
  8. Cifuentes LA, Fogel ML, Pennock JR, Sharp JH (1989) Biogeochemical factors that influence the stable nitrogen isotope ratio of dissolved ammonium in the Delaware Estuary. Geochim Cosmochim Acta 53: 2713–2721Google Scholar
  9. Freyer HD (1978) Seasonal rends of NH4+ and NO3 nitrogen isotopes composition in rain collected at Jülich, Germany. Tellus 30: 83–92Google Scholar
  10. Fry B, Brand W, Mersch FJ, Tholke K, Garritt R (1992) Automated analysis system for coupled δ13C and δ15N measurements. Anal Chem 64: 288–291Google Scholar
  11. Garten CT (1992) Nitrogen isotope composition of ammonium and nitrate in bulk precipitation and forest throughfall. Int J Environ Anal Chem 7: 33–45Google Scholar
  12. Garten C (1993) Variation in foliar 15N abundance and the availability of soil nitrogen on Walker Branch Watershed. Ecology 74: 2098–2113Google Scholar
  13. Giblin AE, Nadelhoffer KJ, Shaver GR, Laundre J, McKerrow A (1991) Biogeochemical diversity along a topographic gradient in a tundra landscape. Ecol Monogr 61: 415–436Google Scholar
  14. Giblin AE, Laundre JA, Nadelhoffer KJ, Shaver GR (1994) Measuring nutrient availability in arctic soils using ion exchange resins: a field test. Soil Sci Soc Am J 58: 1154–1162Google Scholar
  15. Haynes RJ, Goh KM (1978) Ammonium and nitrate nutrition of plants. Biol Rev 53: 465–510Google Scholar
  16. Heaton THE (1987) 15N/14N ratios of nitrate and ammonium in rain at Pretoria, South Africa. Atmos Environ 21: 843–852Google Scholar
  17. Ingestad T (1973) Mineral nutrient requirements of Vaccinium vitus-idaea and V. myrtillus. Physiol Plant 29: 239–246Google Scholar
  18. Kielland K (1994) Amino acid absorption by arctic plants: implications for plant nutrition and nitrogen cycling. Ecology 75: 2373–2383Google Scholar
  19. Kielland K, Chapin FS III (1992). Nutrient absorption and accumulation in arctic plants. In: Chapin FS III, Jefferies R, Reynolds JF, Shaver GR, Svoboda J (eds) Arctic ecosystems in a changing climate: an ecophysiological perspective. Academic Press, San Diego, pp 321–335Google Scholar
  20. Kummerow J, Elliss BA, Kummerow S, Chapin FS (1983) Spring growth of shoots and roots in shrubs of an Alaskan muskeg. Am J Bot 70: 1509–1515Google Scholar
  21. Mariotti A, Pierre D, Vedy JC, Bruckert S, Guillemot J (1980) The abundance of natural nitrogen-15 in the organic matter of soils along an altitudinal gradient. Catena 7: 293–300Google Scholar
  22. Mariotti A, Lancelot C, Billen G (1984) Natural isotopic composition of nitrogen as a tracer of origin for suspended organic matter in the Scheldt estuary. Geochim Cosmochim Acta 48: 549–555Google Scholar
  23. McKane RB, Grigal DF, Russelle MP (1990) Spatiotemporal differences in 15N uptake and the organization of an old-field plant community. Ecology 71: 1126–1183Google Scholar
  24. Michelsen A, Schmidt IK, Jonasson S, Quarmby C, Sleep D (1996) Leaf 15N abundance of subarctic plants provides field evidence that ericoid, ectomycorrhizal and non- and arbuscular mycorrhizal species access different sources of soil nitrogen. Oecologia 105: 53–63Google Scholar
  25. Montoya JP, Wiebe PH, McCarthy JJ (1991) Rapid, storm-induced changes in the natural abundance of 15N in a planktonic ecosystem. Geochim Cosmochim Acta 55: 3627–3638Google Scholar
  26. Nadelhoffer KJ, Fry B (1988) Controls on natural nitrogen-15 and carbon-13 abundances in forest soil organic matter. Soil Sci Soc Am J 52: 1633–1640Google Scholar
  27. Nadelhoffer KJ, Fry B (1994) Nitrogen isotope studies in forest ecosystems. In: Lajtha K, Michener R (eds) Stable isotopes in ecology. Blackwell, Oxford, pp 22–44Google Scholar
  28. Nadelhoffer KJ, Giblin AE, Shaver GR, Linkins AE (1992) Microbial processes and plant nutrient availability in arctic soils. In: Chapin FS III, Jefferies RL, Reynolds JF, Shaver GR, Svoboda J (eds) Physiological ecology of arctic plants: implications for climate change. Academic Press, New York, pp 281–300Google Scholar
  29. Read DJ (1994) Plant-microbe mutualisms and community structure. In: Schulze E-D, Mooney HA (eds) Biodiversity and ecosystem structure. Springer, Berlin Heidelberg New York, pp 181–209Google Scholar
  30. Schulze E-D, Chapin FS III, Gebauer G (1994) Nitrogen nutrition and isotope differences among life forms at the northern treeline of Alaska. Oecologia 100: 406–412Google Scholar
  31. Shaver GR (1995) Plant functional diversity and resource control of primary production in Alaskan arctic tundras. In: Chapin FS III, Körner C (eds) Arctic and alpine biodiversity. Springer, Berlin Heidelberg New York, pp 199–211Google Scholar
  32. Shaver GR, Billings WD (1975) Root production and root turnover in a wet tundra ecosystem, Barrow, Alaska. Ecology 56: 401–409Google Scholar
  33. Shaver GR, Chapin FS III (1991) Production/biomass relationships and element cycling in contrasting arctic vegetation types. Ecol Monogr 61: 1–31Google Scholar
  34. Shaver GR, Chapin FS III (1995) Long term responses to factorial NPK fertilizer treatment by Alaskan wet and moist tundra sedge species. Ecography, in pressGoogle Scholar
  35. Shaver G, Cutler JC (1979) The vertical distribution of phytomass in cottongrass tussock tundra. Arct Alp Res 11 (3): 335–342Google Scholar
  36. Shaver GR, Kummerow J (1992) Phenology, resource allocation, and growth of arctic vascular plants. In: Chapin FS III, Jefferies RL, Reynolds JF, Shaver GR, Svoboda J (eds) Arctic ecosystems in a changing climate: an ecophysiological perspective. Academic Press, New York, pp 193–212Google Scholar
  37. Shaver GR, Fetcher N, Chapin FS III (1986) Growth and flowering in Eriophorum vaginatum: Annual and latitudinal variation. Ecology 67: 1524–1535Google Scholar
  38. Shaver GR, Nadelhoffer KJ, Giblin AE (1991) Biogeochemical diversity and element transport in a heterogeneous landscape, the North Slope of Alaska. In: Turner M, Gardner R (eds) Quantitative methods in landscape ecology. Springer, Berlin Heidelberg New York, pp 105–125Google Scholar
  39. Shearer G, Kohl D (1989) Estimates of N2 fixation in ecosystems: the need for and basis of the 15N natural abundance method. In: Rundel RW, Ehleringer JR, Nagy KA (eds) Stable isotopes in ecological research. Springer, Berlin Heidelberg New York, pp 342–374Google Scholar
  40. Shearer G, Duffy J, Kohl DH, Commoner B (1974) A steady-state model of isotopic fractionation accompanying nitrogen transformations in soil. Soil Sci Soc Am Proc 38: 315–322Google Scholar
  41. Stewart FM, Levin BR (1973) Partitioning of resources and the outcome of interspecific competition: a model and some general considerations. Am Nat 107: 171–198Google Scholar
  42. Stribley DB, Read DJ (1980) The biology of mycorrhizae in the Ericaceae. VII. The relationship between mycorrhizal infection and the capacity to utilize simple and complex organic nitrogen sources. New Phytol 86: 365–371Google Scholar
  43. Tilman D (1988) Plant strategies and the dynamics and structure of plant communities. Princeton University Press, Princeton, New JerseyGoogle Scholar
  44. Vitousek PM, Shearer G, Kohl DH (1989) Foliar 15N natural abundance in Hawaiian rainforest: patterns and possible mechanisms. Oecologia 78: 383–388Google Scholar
  45. Wada E, Hattori A (1978) Nitrogen isotope effects in the assimilation of inorganic nitrogenous compounds. Geomicrobiol J 1: 85–101Google Scholar
  46. Walker DA (1985) Vegetation and environmental gradients of the Prudhoe Bay region. Alaska US Army Cold Regions Research and Engineering Laboratory report no 85-14, US Army Corps of Engineers, 234 ppGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Nadelhoffer
    • 1
  • G. Shaver
    • 1
  • B. Fry
    • 1
  • A. Giblin
    • 1
  • L. Johnson
    • 1
  • R. McKane
    • 1
  1. 1.Marine Biological LaboratoryThe Ecosystems CenterWoods HoleUSA

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