Chromosoma

, Volume 91, Issue 3–4, pp 251–258 | Cite as

Immunofluorescence localization of DNA:RNA hybrids in Drosophila melanogaster polytene chromosomes

  • Inna E. Vlassova
  • Galima H. Umbetova
  • Vladimir H. Zimmermann
  • Carlos Alonso
  • Elena S. Belyaeva
  • Igor F. Zhimulev
Article

Abstract

Sites of transcriptional activity in the whole set of Drosophila melanogaster polytene chromosomes have been localized by means of fluorescent antibodies against DNA:RNA hybrid molecules and compared with results on 3H-uridine incorporation obtained earlier. — The majority of large and small puffs with intensive 3H-uridine incorporation demonstrate bright fluorescence. Moreover, bright fluorescence is also observed for a large number of small puffs though the intensity of 3H-uridine incorporation is low. Some prominent puffs with high levels of 3H-uridine incorporation show weak fluorescence. Condensed bands, as a rule, do not show fluorescence. — The regions that look like interbands under the light microscope are not real interbands, but consist of minibands visible only in the electron microscope (EM). However, a region that has been previously studied by EM and proven to be a real interband between two thick dark bands (100B3-100B4-5) showed fluorescence. These data support previous suggestions indicating a subsantial contribution of transcriptional products from small puffs and interbands to the whole transcriptional system of polytene chromosomes.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Inna E. Vlassova
    • 2
  • Galima H. Umbetova
    • 2
  • Vladimir H. Zimmermann
    • 2
  • Carlos Alonso
    • 1
  • Elena S. Belyaeva
    • 2
  • Igor F. Zhimulev
    • 2
  1. 1.Universidad Autonoma de MadridMadridSpain
  2. 2.Institute of Cytology and GeneticsNovosibirskUSSR

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