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Molecular and General Genetics MGG

, Volume 208, Issue 3, pp 412–419 | Cite as

The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO

  • Yumiko Sano
  • Makoto Kageyama
Article

Summary

The recA gene of Pseudomonas aeruginosa has been isolated and its nucleotide sequence has been determined. The coding region of the recA gene has 1038 bp specifying 346 amino acids. The recA protein of P. aeruginosa showed a striking homology with that of Escherichia coli except for the carboxy-terminal region both at the nucleotide and amino acid level. The recA+-carrying plasmids restored the UV sensitivity and recombination ability of several rec mutants of P. aeruginosa. The precise location of the recA gene on the chromosome was deduced from the analysis of R′ plasmids.

Key words

P. aeruginosa recA Nucleotide sequence 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Yumiko Sano
    • 1
  • Makoto Kageyama
    • 1
  1. 1.Mitsubishi-Kasei Institute of Life SciencesMachida, TokyoJapan

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