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Molecular and General Genetics MGG

, Volume 196, Issue 2, pp 339–344 | Cite as

The transformation of mycelial spheroplasts of Neurospora crassa and the attempted isolation of an autonomous replicator

  • F. P. Buxton
  • A. Radford
Article

Summary

A new transformation protocol for mycelially derived spheroplasts of Neurospora was developed and optimised for a number of components. This protocol was used to screen a bank of 700 clones for high frequency transforming plasmids using miniscreen DNA. Four with significantly increased frequency were found, but they were not capable of autonomous replication. We discuss the conditions that an autonomously replicating plasmid must meet in order to exist in a mycelial fungus such as Neurospora.

Keywords

Mycelial Fungus Frequency Transforming Neurospora Crassa Transformation Protocol Transforming Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • F. P. Buxton
    • 1
  • A. Radford
    • 1
  1. 1.Department of GeneticsUniversity of LeedsLeedsEngland

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