Molecular and General Genetics MGG

, Volume 196, Issue 2, pp 332–338 | Cite as

Temperature sensitive allosuppressor mutants of the fission yeast S. pombe influence cell cycle control over mitosis

  • Paul Nurse
  • Pierre Thuriaux
Article

Summary

A collection of Schizosaccharomyces pombe mutants has been obtained which restore activity to a nonsense suppressing tRNA sup3–5 whose suppressing function has been inactivated by second site mutations within the sup3–5 gene. These mutants were screened for those that were temperature sensitive in suppressing the opal nonsense allele ade6-704. Some of these map within or close to sup3 itself and others define two allosuppressor genes sal2 and sal3. The temperature sensitive mutants fail to efficiently suppress any other opal nonsense alleles although one mutant, sup3–5, r57, rr2, weakly does so at the low temperature. sal2 and sal3 mutants have a pleiotropic effect on the cell cycle causing a transient or complete blockage of mitosis. This blockage and the allosuppressor phenotypes are both eliminated by the presence of wee mutations in wee1 or cdc2. Mutants in sal2 are allelic with cdc25, a gene required for successful completion of mitosis. It is suggested that sal3 and cdc25 influence the mechanism that links the growth rate of the cell with the initiation of mitosis. Mutants in these genes may disturb tRNA biosynthesis or protein synthesis and this disruption may have an effect on both nonsense suppression and the growth rate control over mitosis.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barber H (1966) Allelespetische Suppressormutationen von Schizosaccharomyces pombe. Genetica 37: 109–148Google Scholar
  2. Cox BS (1977) Allosuppressors in yeast. Genet Res 30: 187–204Google Scholar
  3. Di Caprio L, Hastings PJ (1976) Gene conversion and intragenic recombination at the sup6 locus and the surrounding region in Saccharomyces cereviseae. Genetics 87: 697–721Google Scholar
  4. Egel R, Kohli J, Thuriaux P, Wolf K (1980) Genetics of the fission yeast Schizosaccharomyces pombe. Annu Rev Genet 14: 77–108Google Scholar
  5. Fantes P, Nurse P (1977) Control of cell size at division in fission yeast by a growth modulated site control over nuclear division. Exp Cell Res 107: 377–386Google Scholar
  6. Fantes P, Nurse P (1978) Control of the timing of cell division in fission yeast. Exp Cell Res 115: 317–329Google Scholar
  7. Fantes P (1979) Epistatic gene interactions in the control of division in fission yeast. Nature 279: 428–430Google Scholar
  8. Gorman JA, Gorman J (1971) Genetic analysis of a gene required for the expression of allele specific suppression in Saccharomyces cerevisiae. Genetics 67: 337–352Google Scholar
  9. Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RJ (ed) Handbook of genetics, vol 1. Plenum Press, New York, pp 395–446Google Scholar
  10. Gygax A, Thuriaux P (1984) A revised chromosome map of the fission yeast Schizosaccharomyces pombe. Current Genet 8: 85–92Google Scholar
  11. Heyer WD, Thuriaux P, Kohli J, Ebert P, Kersten H, Oehrke C, Kuo KC, Agris PF (1984) An antisuppressor mutation of Schizosaccharomyces pombe affects the post transcriptional modification of the wobble basis in the anticodon of tRNA's. J Biol Chem 259: 2856–2862Google Scholar
  12. Hofer F, Hollenstein H, Janner F, Minet M, Thuriaux P, Leupold U (1979) The genetic fine structure of nonsense suppression in Schizosaccharomyces pombe. Current Genet 1: 45–61Google Scholar
  13. Hopper AK, Schulz LD, Shapiro RA (1980) Processing of intervening sequences: A new yeast mutant which fails to excise intervening sequences from precursor tRNA. Cell 19: 741–751Google Scholar
  14. Hottinger H, Pearson D, Yamao F, Gamulin V, Colley L, Cooper T, Soll D (1982) Nonsense suppression in Schizosaccharomyces pombe: the S. pombe sup3-e tRNA gene is active in S. cerevisiae. Mol Gen Genet 188: 219–224Google Scholar
  15. Janner F, Vogeli G, Fluri R (1980) The effect of an antisuppressor on tRNA in the yeast Schizosaccharomyces pombe. J Mol Biol 139: 207–219Google Scholar
  16. Kohli J, Hottinger H, Munt P, Strauss A, Thuriaux P (1977) Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidisation. Genetics 87: 471–489Google Scholar
  17. Kurjan J, Hall BD, Gillam S, Smith N (1980) Mutation at the yeast tRNAtyr locus: DNA sequences changes in mutants lacking suppressor activity. Cell 20: 701–709Google Scholar
  18. Laten H, Gorman J, Bock RM (1978) Isopentanyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae. Nucl Acids Res 5: 4329–4342Google Scholar
  19. MacCready S, Cox BS (1979) Antisuppressors in yeast. Mol Gen Genet 124: 305–320Google Scholar
  20. Mitchison JM (1970) Physiological and cytological methods for Schizosaccharomyces pombe. Methods Cell Physiol 4: 131–165Google Scholar
  21. Munz P, Leupold U (1979) Gene conversion in nonsense suppressors of Schizosaccharomyces pombe. Mol Gen Genet 170: 15–148Google Scholar
  22. Munz P, Leupold U (1980) Heterologous recombination between redundant tRNA genes in Schizosaccharomyces pombe. Alfred Benson Symposium (eds von Wettstein D, Friis J, Kielland-Brandt M, Steuderup A) vol 16. Munksgaard, Kopenhagen, pp 264–275Google Scholar
  23. Nasmyth K, Nurse P (1981) Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 182: 119–124Google Scholar
  24. Nurse P (1975) Genetic control of cell site at cell division in yeast. Nature 256: 547–551Google Scholar
  25. Nurse P, Thuriaux P, Nasmyth K (1976) Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 146: 167–178Google Scholar
  26. Rafalski A, Kohli J, Agris P, Soll D (1979) The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucl Acids Res 6: 2683–2695Google Scholar
  27. Rothstein RJ (1977) A genetic fine structure analysis of the suppressor 3 locus of Saccharomyces cerevisiae. Genetics 85: 55–64Google Scholar
  28. Sherman F (1982) Suppression in the yeast Saccharomyces cerevisiae. In: Strathern JN, Jones EW, Briach JR (eds) The molecular biology of the yeast Saccharomyces cerevisiae, vol 2. pp 463–486Google Scholar
  29. Thuriaux P, Minet M, Hofer F, Leupold U (1975) Genetic analysis of anti-suppressor mutants in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 142: 251–261Google Scholar
  30. Thuriaux P, Nurse P, Carter B (1978) Mutants altered in the control coordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 161: 215–220Google Scholar
  31. Thuriaux P, Sipiczki M, Fantes P (1980) Genetical analysis of a sterile mutant by protoplast fusion in the fission yeast Schizosaccharomyces pombe. J Gen Microbiol 116: 525–528Google Scholar
  32. Thuriaux P, Heyer WD, Strauss A (1982) Organisation of the complex locus trp1 in the fission yeast Schizosaccharomyces pombe. Current Genet 6: 13–18Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Paul Nurse
    • 1
    • 2
  • Pierre Thuriaux
    • 3
  1. 1.School of Biological SciencesUniversity of SussexBrightonUK
  2. 2.Department of ZoologyUniversity of EdinburghEdinburghUK
  3. 3.Institut für Allgemeine Mikrobiologie der Universität BernBernSwitzerland

Personalised recommendations