World Journal of Microbiology and Biotechnology

, Volume 12, Issue 6, pp 615–617 | Cite as

Characteristics of extracellular proteases produced by Bacillus laterosporus and Flavobacterium sp. isolated from gelatinfactory effluents

  • A. Sharma
  • C. L. S. N. Rao
  • B. K. Ball
  • S. K. Hasija
Research

Abstract

Forty bacterial isolates from the effluents of a gelatin factory (Jabalpur, India) were screened for protease activity and the two most potent producers were identified as Bacillus laterosporus and a Flavobacterium sp. The enzymes of both isolates were optimal at pH 8 and 60°C, with maximum activity after 90 min. The enzyme activity of B. laterosporus was suppressed by Fe2+, Mg2+, Mn2+ and Zn2+ ions but was enhanced by Ba2+ and Ca2+. That of Flavobacterium sp. was suppressed by Mg2+ and Mn2+ ions but enhanced by Ba2+, Ca2+ and Fe2+. The enzyme activity of the former was strongly inhibited by KCN, whereas that of the latter was only slightly inhibited by 8-hydroxyquinoline.

Key words

Bacillus laterosporus Flavobacterium gelatin protease 

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References

  1. Anon. 1985 Standard Methods for Examination of Water and Waste Water, 16th edn. New York: American Public Health Association.Google Scholar
  2. Bryant, T.N. 1989 Probable Identification of Bacteria. Southampton, UK: Medical Statistics and Computing.Google Scholar
  3. Buchanan, R.E. & Gibbons, N.E. (eds) 1974 Bergey's Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins.Google Scholar
  4. Chakraborty, R. & Srinivasan, M. 1992 Production and regulation of a thermostable protease by Pseudomonas sp. B45. Acta Microbiologica Hungarica 39, 181–191.Google Scholar
  5. Chrost, R.J., Halemejko, G.Z. & Overbeck, J. 1986 Is proteolysis dependent on phosphorus in fresh water? FEMS Microbiology Letters 37, 199–202.Google Scholar
  6. Fujio, Y. & Kume, S. 1991 Characteristics of a highly thermostable neutral protease produced from Bacillus stearothermophilus. World Journal of Microbiology & Biotechnology 7, 12–16.Google Scholar
  7. Jacob, M.B. & Gerstein, M.J. 1960 Handbook of Microbiology. New York: Van Nostrand, Reinhold.Google Scholar
  8. Kubo, M., Okajima, J. & Hasumi, F. 1994 Isolation and characterization of soybean waste-degrading microorganisms and analysis of fertilizer effects of the degraded products. Applied and Environmental Microbiology 60, 343–347.Google Scholar
  9. Kunitz, M. 1947 Crystalline soybean trypsin inhibition. Journal of General Physiology 30, 291–320.Google Scholar
  10. Marrink, J. & Gruber, M. 1966 Proteolytic activity in tissue extracts. Biochemica et Biophysica Acta 118, 438–451.Google Scholar
  11. Michotey, V. & Blanco, C. 1994 Characterization of an endoserine protease secreted by Arthrobacter aureus. Applied and Environmental Microbiology 60, 341–343.Google Scholar
  12. Priest, F.G. 1977 Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews 41, 711–753.Google Scholar
  13. Sen, S. & Satyanarayana, T. 1993 Optimization of alkaline protease production by thermophilic Bacillus licheniformis S-40. Indian Journal of Microbiology. 31, 43–47.Google Scholar

Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • A. Sharma
    • 1
  • C. L. S. N. Rao
    • 1
  • B. K. Ball
    • 1
  • S. K. Hasija
    • 1
  1. 1.Bacteriology Laboratory, Department of Biological SciencesRani Durgavati UniversityJabalpurIndia

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