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Chromosoma

, Volume 59, Issue 1, pp 13–22 | Cite as

DNA reassociation kinetics in relation to genome size in four amphibian species

  • Cosima T. Baldari
  • Francesco Amaldi
Article

Abstract

DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Trituras cristatus (23 pg) and Necturus maculosus (52 pg) of the Urodela subclass.

Within each subclass the two species studied were found to have about the same absolute amount of unique DNA. The differences of total nuclear DNA can be accounted for by quantitative variations of the repetitive sequence classes, at least in part due to changes in the number of copies of the various sequences. On the contrary the great difference in nuclear DNA between the two subclasses, Anura and Urodela, involves all sequence classes in parallel; the slowly reassociating fraction appears to be unique in spite of a tenfold difference in absolute amount.

The dependence of reassociation kinetics on DNA fragment length for the four species indicates for all of them an interspersed organization of the various sequence classes.

Keywords

Hydroxyapatite Genome Size Repetitive Sequence Xenopus Laevis Absolute Amount 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Amaldi, F., Lava-Sanchez, P.A., Buongiorno-Nardelli, M.: Redundancy of genetic information and evolution in living systems. Rend. Ace. Naz. Lincei S 8; 58, 939–944 (1975)Google Scholar
  2. Britten, R.J., Kohne, D.E.: Repeated Sequences in DNA. Science 161, 529–540 (1968)Google Scholar
  3. Britten, R.J., Davidson, E.H.: Gene regulation for higher cells: a theory. Science 165, 349–357 (1969)Google Scholar
  4. Buongiorno-Nardelli, M., Amaldi, F., Lava-Sanchez, P.A.: Amplification as a rectification mechanism for the redundant rRNA genes. Nature (Lond.) New Biol. 238, 134–137 (1972)Google Scholar
  5. Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with nonrepetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)Google Scholar
  6. Davis, R., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzimology (L. Grossman and K. Moldave, eds.) 21, 413–428. New York: Academic Press 1971Google Scholar
  7. Flavell, R.B., Bennet, M.D., Smith, J.B., Smith, D.B.: Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269 (1974)Google Scholar
  8. Hinegardner, R., Rosen, D.E.: Cellular DNA content and the evolution of Teleostean Fishes. Amer. Naturalist 106, 621–644 (1972)Google Scholar
  9. Mizuno, S., Macgregor, H.C.: Chromosomes, DNA sequences and evolution in salamanders of the genus Plethodon. Chromosoma (Berl.) 48, 239–296 (1974)Google Scholar
  10. Morescalchi, A., Serra, V.: DNA renaturation kinetics in some paedogenetic Urodeles. Experientia (Basel) 30, 487–89 (1974)Google Scholar
  11. Morescalchi, A.: Amphibia. In: Cytotaxonomy and vertebrate evolution (A.B. Chiarelli and E. Capanna, eds.), pp. 233–348. London and New York: Academic Press 1973Google Scholar
  12. Narayan, R.K.J., Rees, H.: Nuclear DNA variation in Lathyrus. Chromosoma (Berl.) 54, 141–154 (1976)Google Scholar
  13. Ohno, S., Wolf, U., Atkin, N.B.: Evolution from Fishes to Mammals by gene duplication. Hereditas (Lund) 59, 169–187 (1968)Google Scholar
  14. Olmo, E.: Quantitative variations in the nuclear DNA and phylogenesis of the Amphibia. Caryologia (Firenze) 26, 43–68 (1973)Google Scholar
  15. Straus, N.A.: Comparative DNA renaturation kinetics in Amphibians. Proc. nat. Acad. Sci. (Wash.) 68, 799–802 (1971)Google Scholar
  16. Studier, S.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)Google Scholar
  17. Wetmur, J.G., Davidson, N.: Kinetics of renaturation of DNA. J. molec. Biol. 31, 349–370 (1968)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Cosima T. Baldari
    • 1
  • Francesco Amaldi
    • 1
  1. 1.Centro di Studi per gli Acidi Nucleici, C.N.R., Istituto di Fisiologia GeneraleUniversità di Roma, Città UniversitariaRomaItaly

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