, Volume 59, Issue 1, pp 1–12 | Cite as

Contrasting patterns of DNA sequence arrangement in Apis mellifera (Honeybee) and Musca domestica (housefly)

  • William R. Crain
  • Eric H. Davidson
  • Roy J. Britten


We have examined the organization of the repeated and single copy DNA sequences in the genomes of two insects, the honeybee (Apis mellifera) and the housefly (Musca domestica). Analysis of the reassociation kinetics of honeybee DNA fragments 330 and 2,200 nucleotides long shows that approximately 90% of both size fragments is composed entirely of non-repeated sequences. Thus honeybee DNA contains few or no repeated sequences interspersed with nonrepeated sequences at a distance of less than a few thousand nucleotides. On the other hand, the reassociation kinetics of housefly DNA fragments 250 and 2,000 nucleotides long indicates that less than 15% of the longer fragments are composed entirely of single copy sequences. A large fraction of the housefly DNA therefore contains repeated sequences spaced less than a few thousand nucleotides apart. Reassociated repetitive DNA from the housefly was treated with S1 nuclease and sized on agarose A-50. The S1 resistant sequences have a bimodal distribution of lengths. Thirty-three percent is greater than 1,500 nucleotide pairs, and 67% has an average size about 300 nucleotide pairs. The genome of the housefly appears to have at least 70% of its DNA arranged as short repeats interspersed with single copy sequences in a pattern qualitatively similar to that of most eukaryotic genomes.


Repeated Sequence Apis Mellifera Size Fragment Bimodal Distribution Eukaryotic Genome 
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  1. Angerer, R.C., Davidson, E.H., Britten, R.J.: DNA sequence organization in the mollusc Aplysia californica. Cell 6, 29–39 (1975)Google Scholar
  2. Bier, K., Müller, W.: DNS-Messungen bei Insekten und eine Hypothese über retardierte Evolution und besonderen DNS-Reichtum im Tierreich. Biol. Zbl. 88, 425–449 (1969)Google Scholar
  3. Britten, R.J., Davidson, E.H.: Gene regulation for higher cells: a theory. Science 165, 349–357 (1969)Google Scholar
  4. Britten, R.J., Davidson, E.H.: DNA sequence arrangement and preliminary evidence on its evolution. FASEB 35, 2151–2157 (1976)Google Scholar
  5. Britten, R.J., Graham, D.E., Eden, F.C., Painchaud, D.M., Davidson, E.H.: Evolutionary divergence and length of repetitive sequences in sea urchin DNA. J. molec. Evol. (in press, 1976)Google Scholar
  6. Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), vol. 29, part E, p. 363–418. New York: Academic Press 1974Google Scholar
  7. Britten, R.J., Kohne, D.: Repeated sequences in DNA. Science 161, 529–540 (1968)Google Scholar
  8. Crain, W.R., Eden, F.C., Pearson, W.R., Davidson, E.H., Britten, R.J.: Absence of short period interspersion of repetitive and nonrepetitive sequences in the DNA of Drosophila melanogaster. Chromosoma (Berl.) 56, 309–326 (1976)Google Scholar
  9. Davidson, E.H., Galau, G.A., Angerer, R.C., Britten, R.J.: Comparative aspects of DNA sequence organization in metazoa. Chromosoma (Berl.) 51, 253–259 (1975b)Google Scholar
  10. Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with nonrepetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)Google Scholar
  11. Davidson, E.H., Hough, B.R., Klein, W.H., Britten, R.J.: Structural genes adjacent to interspersed repetitive DNA sequences. Cell 4, 217–238 (1975a)Google Scholar
  12. Efstradiatis, A., Crain, W.R., Britten, R.J., Davidson, E.H.: DNA sequence organization in the lepidopteran Antheraea pernyi. Proc. nat. Acad. Sci. (Wash.) 73, 2289–2293 (1976)Google Scholar
  13. Firtel, R.A., Kindle, K.: Structural organization of the genome of the cellular slime mold Dictyostelium discoideum: interspersion of repetitive and single-copy DNA sequences. Cell 5, 401–411 (1975)Google Scholar
  14. Fristrom, J.W., Yund, M.A.: Genetic programming for development in Drosophila. Crit. Rev. Biochem. 1, 537–570 (1973)Google Scholar
  15. Goldberg, R.B., Crain, W.R., Ruderman, J.V., Moore, G.P., Barnett, T.R., Higgins, R.C., Gelfand, R.A., Galau, G.A., Britten, R.J., Davidson, E.H.: DNA sequence organization in the genomes of five marine invertebrates. Chromosoma (Berl.) 51, 225–251 (1975)Google Scholar
  16. Graham, D.E., Neufeld, B.R., Davidson, E.H., Britten, R.J.: Interspersion of repetitive and nonrepetitive DNA sequences in the sea urchin genome. Cell 1, 127–138 (1974)Google Scholar
  17. Jordan, R.A., Brosemer, R.W.: Characterization of DNA from three bee species. J. Insect Physiol. 20, 2513–2520 (1974)Google Scholar
  18. Manning, J.E., Schmid, C.W., Davidson, N.: Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)Google Scholar
  19. Wells, R., Royer, H.D., Hollenberg, C.P.: Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. (in press, 1976)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • William R. Crain
    • 1
    • 2
  • Eric H. Davidson
    • 2
  • Roy J. Britten
    • 2
    • 3
  1. 1.The Worcester Foundation for Experimental BiologyShrewsburyUSA
  2. 2.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA
  3. 3.Carnegie Institution of WashingtonWashington, D.C.USA

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