Molecular and General Genetics MGG

, Volume 192, Issue 1–2, pp 288–289 | Cite as

Insertion mutations in the dam gene of Escherichia coli K-12

  • M. G. Marinus
  • Margaretha Carraway
  • Alexis Z. Frey
  • Leif Brown
  • Judy A. Arraj
Article

Summary

The dam gene of E. coli can be inactivated by insertion of Tn9 or Mud phage. Strains bearing these mutations are viable indicating that the dam gene product is dispensable.

References

  1. Arraj JA, Marinus MG (1983) Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam + gene. J Bacteriol 153:562–565Google Scholar
  2. Bale A, D'Alarcao M, Marinus MG (1979) Characterization of DNA adenine methylation mutants of Escherichia coli K-12. Mutation Res 59:157–165Google Scholar
  3. Brooks JE, Blumenthal RM, Gingeras TR (1983) The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res 11:837–851Google Scholar
  4. Casadaban MJ, Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76:4530–4533Google Scholar
  5. Hattman S (1982) DNA methyltransferase-dependent transcription of the phage Mu mom gene. Proc Natl Acad Sci USA 79:5518–5521Google Scholar
  6. Lacks S, Greenberg B (1977) Complementary specificty of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol 144:153–168Google Scholar
  7. Lark C (1968) Effect of the methionine analogues ethionine and norleucine on DNA synthesis in Escherichia coli 15T. J Mol Biol 31:401–414Google Scholar
  8. Marinus MG (1973) Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet 127:47–55Google Scholar
  9. Marinus MG, Morris NR (1973) Isolation of DNA methylase mutants of Escherichia coli K-12. J Bacteriol 114:1143–1150Google Scholar
  10. Meijer M, Beck E, Hansen FC, Bergmans HEN, Messer W, von Meyenberg K, Schaller H (1979) Nucleotide sequence of the origin of replication of the Escherichia coli K-12 chromosome. Proc Natl Acad Sci USA 76:580–584Google Scholar
  11. Plasterk RHA, Vrieling H, van de Putte P (1983) Transcription initiation of the Mu mom depends on methylation of the promoter region and a phage-coded transactivator. Nature 301:344–347Google Scholar
  12. Sugimoto K, Oka A, Sugisaki M, Takanami M, Nishimura A, Yasuda Y, Hirota Y (1979) Nucleotide sequence of Escherichia coli K-12 replication origin. Proc Natl Acad Sci USA 76:575–579Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • M. G. Marinus
    • 1
  • Margaretha Carraway
    • 1
  • Alexis Z. Frey
    • 1
  • Leif Brown
    • 1
  • Judy A. Arraj
    • 1
  1. 1.Department of PharmacologyUniversity of Massachusetts Medical SchoolWorcesterUSA

Personalised recommendations