Molecular and General Genetics MGG

, Volume 210, Issue 3, pp 504–508

The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated

  • Deborah R. Gill
  • George P. C. Salmond
Article

Summary

The cell division genes ftsY, ftsE and ftsX form an operon mapping at 76 min on the Escherichia coli chromosome. The protein products of these genes have been indentified previously. We have studied the cellular location of the radiolabelled Fts proteins using maxicells and standard fractionation procedures. Previous protein sequence homologies suggested an inner membrane location for FtsE. We have confirmed this predicted location and have shown that FtsY and FtsX are also inner membrane-associated. These results are igreement with the hypothesis that FtsE may act at the inner membrane, in a “septalsome” complex, by coupling ATP hydrolysis to the process of bacterial cell division.

Key words

Escherichia coli Cell division ftsE Operon Membrane location 

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References

  1. Ames GF-L, Nikaido K (1978) Identification of a membrane protein as a histidine transport component in Salmonella typhimurium. Proc Natl Acad Sci USA 75:5447–5451Google Scholar
  2. Bavoil, P, Hofnung M, Nikaido K (1980) Identification of a cytoplasmic membrane-associated component of the maltose transport system of E. coli. J Biol Chem 255:8366–8369Google Scholar
  3. Bremer E, Beck E, Hindennach I, Sonntag I, Henning U (1980) Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K12. Mol Gen Genet 179:13–20Google Scholar
  4. Clement J-M, Perrin D, Hedgpeth J (1982) Analysis of λ receptor and β-lactamase synthesis and export using cloned genes in a minicell system. Mol Gen Genet 185:302–310Google Scholar
  5. Churchward GG, Holland IB (1976) Envelope synthesis during the cell cycle in E. coli B/r. J Mol Biol 105:245–261Google Scholar
  6. Donachie WD, Begg KJ, Sullivan NF (1984) The morphogenes of Escherichia coli. In: ‘Microbial Development’. Losick J, Shapiro L, eds. pp 27–62. Cold Spring Harbor Publications, New YorkGoogle Scholar
  7. Doolittle RF, Johnson MS, Husain I, Van Houten B, Thomas DC, Sancar A (1986) Domainal evolution of a prokaryotic DNA repair protein and its relationship to active transport proteins. Nature 323:451–453Google Scholar
  8. Filip C, Fletcher G, Wulff JL, Earhart CF (1973) Solubilisation of the cytoplasmic membrane of E. coli by the ionic detergent sodium lauryl sarcosinate. J Bacteriol 115:717–722Google Scholar
  9. Friedrich MJ, DeVeaux LC, Kadner RJ (1986) Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in E. coli and homology with components of periplasmic-binding-protein-dependent transport systems. J Bacteriol 167:928–934Google Scholar
  10. Gerlach JH, Endicott JA, Juranka PF, Henderson G, Sarangi F, Deuchars KL, Ling V (1986) Homology between P-glycoprotein and a bacterial haemolysin transport protein suggests a model for multidrug resistance. Nature 324:485–489Google Scholar
  11. Gill DR, Hatfull GF, Salmond GPC (1986) A new cell division operon in Escherichia coli Mol Gen Genet 205:134–145Google Scholar
  12. Halegoua S, Inouye M (1979) Translocation and assembly of outer membrane proteins of E. coli. J Mol Biol 130:39–61Google Scholar
  13. Higgins CF, Hiles ID, Salmond GPC, Gill DR, Downie JA, Evans IJ, Holland IB, Gray L, Buckel SD, Bell AW, Hermodson MA (1986) A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria. Nature 323:448–450Google Scholar
  14. Higgins CF, Hiles ID, Whalley K, Jamieson DJ (1985) Nucleotide binding by membrane components of bacterial periplasmic binding protein-dependent transport system. EMBO J 4:1033–1040Google Scholar
  15. Hobson A, Weatherwax R, Ames GF-L (1984) ATP-binding sites in the membrane components of histidine permease, a periplasmic transport system. Proc Natl Acad Sci 81:7333–7337Google Scholar
  16. Holland IB (1987) Genetic analysis of the E. coli division clock. Cell 48:361–362Google Scholar
  17. Holland IB, Jones CA (1985) The role of the FtsZ protein (SfiB) in UV-induced division inhibition and in the normal E. coli cell division cycle. Ann Inst Pasteur 136:165–171Google Scholar
  18. Jackson ME, Pratt JM, Stoker NG, Holland IB (1985) An inner membrane protein N-terminal signal sequence is able to promote efficient localisation of an outer membrane protein in E. coli. EMBO J 4:2377–2383Google Scholar
  19. Jackson ME, Pratt JM, Holland IB (1986) Intermediates in the assembly of the TonA polypeptide into the outer membrane of E. coli K-12. J Mol Biol 189:477–486Google Scholar
  20. Jones CA, Holland IB (1985) Role of the SulB (FtsZ) protein in division inhibition during the SOS response in E. coli: FtsZ stabilises the inhibitor SulA in maxicells. Proc Natl Acad Sci USA 82:6045–6049Google Scholar
  21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685Google Scholar
  22. Mackman N, Nicaud J-M, Gray L, Holland IB (1985) Identification of polypeptides required for the export of haemolysin 2001 from Escherichia coli. Mol Gen Genet 201:529–536Google Scholar
  23. Mount SM (1987) Sequence similarity. Nature 325:487Google Scholar
  24. Neu HC, Heppel LA (1965) The release of enzymes from E. coli by osmotic shock and during the formation of spheroplasts. J Biol Chem 240:3685–3692Google Scholar
  25. Oliver DB, Beckwith J (1982) Regulation of a membrane component required for protein secretion in E. coli. Cell 30:311–319Google Scholar
  26. Pugsley AP, Schwartz M (1985) Export and secretion of proteins by bacteria. FEMS Microbiol Rev 32:3–38Google Scholar
  27. Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693Google Scholar
  28. Silhavy TJ, Benson SA, Emr SD (1983) Mechanisms of protein localisation. Microbiol Rev 47:313–344Google Scholar
  29. Skinner MK, Griswold MD (1983) Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures. Biochem J 209:281–284Google Scholar
  30. Stoker NG, Pratt JM, Spratt BG (1983) Identification of the rodA gene product of E. coli. J Bacteriol 155:854–859Google Scholar
  31. Surin BP, Rosenberg H, Cox GB (1985) Phosphate-specific transport system of E. coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol 161:189–198Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Deborah R. Gill
    • 1
  • George P. C. Salmond
    • 1
  1. 1.Department of Biological SciencesUniversity of WarwickCoventryUK

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