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Molecular and General Genetics MGG

, Volume 210, Issue 3, pp 419–428 | Cite as

The cytoplasmic ribosomes of Chlamydomonas reinhardtii: Characterization of antibiotic sensitivity and cycloheximide-resistant mutants

  • G. H. Fleming
  • J. E. Boynton
  • N. W. Gillham
Article

Summary

In vitro protein synthesis was used to characterize the antibiotic sensitivity of cytoplasmic ribosomes from wild-type and antibiotic-resistant strains of Chlamydomonas reinhardtii. Cytoplasmic ribosomes from two cycloheximide-resistant mutants, act-1 and act-2, were resistant to the antibiotic in vitro. The alteration effected by the act-1 mutation, which was dominant in diploids, was localized to the large subunit of the cytoplasmic ribosomes, but no ribosomal protein alterations were detected using two-dimensional gel electrophoresis. The act-2 mutation, which was semidominant in diploids, was frequently associated with a charge alteration in the large subunit ribosomal protein (r-protein) cyL38 that segregated independently from the antibiotic-resistant phenotype in crosses.

Key words

Cytoplasmic ribosomes Cycloheximide Chlamydomonas reinhardtii 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • G. H. Fleming
    • 1
  • J. E. Boynton
    • 1
  • N. W. Gillham
    • 2
  1. 1.Department of BotanyDuke UniversityDurhamUSA
  2. 2.Department of ZoologyDuke UniversityDurhamUSA

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