, Volume 64, Issue 2, pp 125–132

Chromosomal differentiation in two species of Aedes and their hybrids revealed by giemsa C-banding

  • Moosa A. Motara
  • Karamjit S. Rai


Giemsa C-banding patterns in two species of mosquitoes, Aedes aegypti and Aedes mascarensis, their hybrids and backcross progeny revealed differences in the sex chromosome pair. In A. aegypti, the female determining or the m chromosome in both males and females shows a conspicuous band in the centromere region and another band in one arm. The male determining or the M chromosome is devoid of any bands. Progeny of crosses involving A. aegypti females and A. mascarensis males showed interesting albeit unexpected results. The intercalary band was suppressed in both sons and daughters. When such F1 sons were backcrossed to A. aegypti females, a proportion of males developed into intersexes. These intersex progeny differed from the normal males in terms of their banding pattern. In the reciprocal cross (A. mascarensis female × A. aegypti male), the F1 and the backcross progeny yielded the expected C-banding patterns. The implications of the reversible expression of the intercalary band on the A. aegypti m chromosome and its relevance to genetic regulation are discussed.


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  1. Bianchi, N.O., Sweet, B.H., Ayres, J.: Heterochromatin in cells derived from Aedes albopictus (Skuse) mosquito tissue culture. Exp. Cell Res. 69, 236–239 (1971)Google Scholar
  2. Chandra, S.H., Brown, S.W.: Chromosome imprinting and the mammalian X chromosome. Nature (Lond.) 253, 165–168 (1975)Google Scholar
  3. Craig, G.B., Jr., VandeHey, R.C.: Genetic variability in Aedes aegypti (Diptera: Culicidae). I. Mutations affecting color pattern. Ann. entomol. Soc. America 55, 47–58 (1962)Google Scholar
  4. Hartberg, W.K., Craig, G.B., Jr.: Reproductive isolation in Stegomyia mosquitoes. I. Sexual isolation between Aedes aegypti and A. mascarensis. Ann. ent. Soc. America 61, 865–870 (1968)Google Scholar
  5. Hartberg, W.K., Craig, G.B., Jr.: Reproductive isolation in Stegomyia mosquitoes. II. Hybrid breakdown between Aedes aegypti and A. mascarensis. Evolution (Lawrence, Kans.) 24, 692–703 (1970)Google Scholar
  6. Hartberg, W.K., Craig, G.B., Jr.: Three new mutants in Aedes mascarensis: Currant-eye, small-antenna and yellow. J. med. Entomol. 11, 447–454 (1974)Google Scholar
  7. Kitzmiller, J.B.: Genetics, cytogenetics, and evolution of mosquitoes. Advanc. Genet. 18, 315–433 (1976)Google Scholar
  8. Mattingly, P.F.: The culicine mosquitoes of the Indo-malayan area. Part VI. London: British Museum (Nat. Hist.) 1966Google Scholar
  9. McClelland, G.A.H., Mamet, R.: Aedes aegypti (L.) and Aedes mascarensis MacGreegor in Mauritius: A case of gene survival following species eradication? Nature (Lond.) 195, 965 (1962)Google Scholar
  10. McDonald, P.T., Rai, K.S.: Correlation of linkage groups with chromosomes in the mosquito Aedes aegypti. Genetics 66, 475–485 (1970)Google Scholar
  11. Newton, M.E., Sothern, D.I., Wood, R.J.: X and Y chromosomes of Aedes aegypti (L.) distinguished by giemsa C-banding. Chromosoma (Berl.) 49, 41–49 (1974)Google Scholar
  12. Rai, K.S.: A comparative study of mosquito karyotypes. Ann. entomol. Soc. America 56, 160–170 (1963)Google Scholar
  13. Rai, K.S.: Further observations on the somatic chromosome cytology of some mosquitoes (Diptera: Culicidae). Ann. entomol. Soc. America 59, 242–246 (1966)Google Scholar
  14. Rai, K.S., Hartberg, W.K.: Aedes. In: Handbook of genetics (R.C. King, ed.), Vol. 3, Chap. 13, pp. 311–345. New York: Plenum 1974Google Scholar
  15. Sager, R., Kitchin, R.: Selective silencing of eukaryotic DNA. Science 189, 426–433 (1975)Google Scholar
  16. Steiniger, G.E., Mukherjee, A.B.: Insect chromosome banding: Technique for G- and Q-banding pattern in the mosquito Aedes albopictus. Canad. J. Genet. Cytol. 17, 241–244 (1975)Google Scholar
  17. Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 304–306 (1972)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Moosa A. Motara
    • 1
  • Karamjit S. Rai
    • 1
  1. 1.Department of BiologyUniversity of Notre DameNotre DameUSA

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